测定血浆中的肠高血糖素以检测儿童乳糜泻

Determination of enteroglucagon in plasma for detection of celiac disease in children.

作者信息

Kilander A F, Stenhammar L, Lindstedt G, Lundberg P A

出版信息

Clin Chem. 1984 Jan;30(1):77-80.

Abstract

To evaluate the plasma enteroglucagon assay as a test for the detection of celiac disease, we have determined basal and postprandial concentrations of enteroglucagon in plasma of children who underwent small-intestinal biopsy because of suspected celiac disease. In the 14 children with untreated celiac disease both basal [81 (SD 33) pmol/L] and postprandial [129 (SD 26) pmol/L] concentrations of enteroglucagon were significantly higher (p less than 0.001) than in the 45 children with other gastrointestinal disorders [24 (SD 9) pmol/L, and 50 (SD 22) pmol/L, respectively] and in the 15 children without gastrointestinal disorders [14 (SD 10) pmol/L, and 35 (SD 8) pmol/L, respectively]. All children with celiac disease had either basal or postprandial plasma enteroglucagon concentrations exceeding the mean + 2 SD of the results for the children with other gastrointestinal disorders. Eight of 10 children with celiac disease in whom both concentrations were measured had increased values for both. In our study the sensitivity for detection of celiac disease was 100% and the specificity 97%. Evidently determination of plasma enteroglucagon concentration is effective in diagnosing celiac disease, thereby improving the selection of patients for small-intestinal biopsy.

摘要

为了评估血浆肠高血糖素检测作为乳糜泻检测方法的效果，我们测定了因疑似乳糜泻而接受小肠活检的儿童血浆中肠高血糖素的基础浓度和餐后浓度。14例未经治疗的乳糜泻患儿的肠高血糖素基础浓度[81（标准差33）pmol/L]和餐后浓度[129（标准差26）pmol/L]均显著高于45例患有其他胃肠道疾病的儿童[分别为24（标准差9）pmol/L和50（标准差22）pmol/L]以及15例无胃肠道疾病的儿童[分别为14（标准差10）pmol/L和35（标准差8）pmol/L]（p<0.001）。所有乳糜泻患儿的血浆肠高血糖素基础浓度或餐后浓度均超过患有其他胃肠道疾病儿童结果的均值+2个标准差。在10例同时检测了两种浓度的乳糜泻患儿中，有8例两种浓度均升高。在我们的研究中，检测乳糜泻的敏感性为100%，特异性为97%。显然，测定血浆肠高血糖素浓度对诊断乳糜泻有效，从而改善了小肠活检患者的选择。