Hatch T P, Allan I, Pearce J H
J Bacteriol. 1984 Jan;157(1):13-20. doi: 10.1128/jb.157.1.13-20.1984.
Significant differences in cysteine-containing proteins and detergent-related solubility properties were observed between outer membrane protein complexes of reproductive (reticulate body) and infective (elementary body) forms of Chlamydia psittaci (6BC). Elementary bodies harvested at 48 h postinfection possessed a 40-kilodalton major outer membrane protein and three extraordinarily cysteine-rich outer membrane proteins of 62, 59, and 12 kilodaltons, all of which were not solubilized by sodium dodecyl sulfate in the absence of thiol reagents. Intracellularly dividing reticulate bodies harvested at 21 h postinfection were severely deficient in the cysteine-rich proteins but possessed almost as much major outer membrane protein as did the elementary bodies. Most of the major outer membrane protein of reticulate bodies was solubilized by sodium dodecyl sulfate and was present in envelopes as monomers, although a proportion formed disulfide-cross-linked oligomers. By 21 to 24 h postinfection, reticulate bodies commenced synthesis of the cysteine-rich proteins which were found in outer membranes as disulfide-cross-linked complexes. The outer membranes of reticulate bodies of Chlamydia trachomatis (LGV434) also were found to be deficient in cysteine-rich proteins and to be more susceptible to dissociation in sodium dodecyl sulfate than were outer membranes of elementary bodies.
在鹦鹉热衣原体（6BC）的生殖型（网状体）和感染型（原体）的外膜蛋白复合物之间，观察到含半胱氨酸的蛋白质和与去污剂相关的溶解性特性存在显著差异。感染后48小时收获的原体具有一种40千道尔顿的主要外膜蛋白和三种分别为62、59和12千道尔顿的富含半胱氨酸的外膜蛋白，在没有硫醇试剂的情况下，所有这些蛋白都不能被十二烷基硫酸钠溶解。感染后21小时收获的细胞内正在分裂的网状体严重缺乏富含半胱氨酸的蛋白质，但拥有几乎与原体一样多的主要外膜蛋白。网状体的大部分主要外膜蛋白可被十二烷基硫酸钠溶解，并以单体形式存在于包膜中，尽管有一部分形成了二硫键交联的寡聚体。到感染后21至24小时，网状体开始合成富含半胱氨酸的蛋白质，这些蛋白质在外膜中以二硫键交联的复合物形式存在。沙眼衣原体（LGV434）网状体的外膜也被发现缺乏富含半胱氨酸的蛋白质，并且比原体的外膜更易在十二烷基硫酸钠中解离。
