Barbour A G, Amano K, Hackstadt T, Perry L, Caldwell H D
J Bacteriol. 1982 Jul;151(1):420-8. doi: 10.1128/jb.151.1.420-428.1982.
Chlamydia trachomatis LGV-434 was grown in HeLa 229 cells. Benzylpenicillin completely inhibited the formation of infectious elementary bodies (EBs) at a concentration of 19 pmol/ml or higher and produced abnormally large reticulate bodies (RBs) in the inclusions at 30 pmol/ml or higher. The possible targets for penicillin in C. trachomatis were three penicillin-binding proteins (PBPs) which were identified in the Sarkosyl-soluble fractions of both RBs and EBs. The apparent subunit molecular weights were 88,000 (PBP 1), 61,000 (BPB 2), and 36,000 (PBP 3). The 50% binding concentrations of [3H]penicillin for PBPs 1 to 3 in EBs and RBs were between 7 and 70 pmol/ml. Such high susceptibility to penicillin was shown by an organism that did not have detectable muramic acid (less than 0.02% by weight) in preparations of either whole cells or sodium dodecyl sulfate-insoluble residues.
沙眼衣原体LGV - 434在HeLa 229细胞中培养。苄青霉素在浓度为19 pmol/ml或更高时完全抑制感染性原体（EBs）的形成，在30 pmol/ml或更高时在包涵体中产生异常大的网状体（RBs）。沙眼衣原体中青霉素的可能作用靶点是三种青霉素结合蛋白（PBPs），它们在RBs和EBs的 Sarkosyl可溶部分中被鉴定出来。其表观亚基分子量分别为88,000（PBP 1）、61,000（BPB 2）和36,000（PBP 3）。EBs和RBs中PBP 1至3的[3H]青霉素50%结合浓度在7至70 pmol/ml之间。在全细胞制剂或十二烷基硫酸钠不溶性残渣中均未检测到胞壁酸（重量百分比小于0.02%）的一种生物体表现出对青霉素如此高的敏感性。
