Morris R E, Saelinger C B
J Histochem Cytochem. 1984 Jan;32(1):124-8. doi: 10.1177/32.1.6690597.
A simple, sensitive method to visualize the binding and internalization of protein ligands by cells in culture is described. A biotinylated toxin was used as ligand, and succinoylated avidin adsorbed onto 5.2 nm gold sols was the electron-dense marker. This method affords direct localization of proteins that are on the cell surface or intracellular without need for techniques that alter membrane integrity.
