Stall A M, Loken M R
J Immunol. 1984 Feb;132(2):787-95.
Hybridomas generated from mice immunized with allotype and H-2-incompatible spleen cells were screened by flow cytometry. Monoclonal antibodies (MAb) to four of the five known specificities of IgD were identified. The location of these specificities on the IgD molecule was determined by the ability of a given MAb to bind to cells stripped of the Fab fragment by trypsin proteolysis. Igh-5.1 (present on IgDa and IgDe) and Igh-5.5 (unique to IgDe) were both located on the Fab fragment. Results from cross-blocking experiments suggest that, except for Igh-5.3, MAb which recognize the same specificity bind to the same antigenic determinant. Both the trypsin digest and blocking studies indicate that Igh-5.3 is composed of at least two antigenic determinants. AF6-78.25, a MAb specific for IgM of the b, d, and n haplotypes, was also identified; this MAb defines a new specificity, Igh-6.6. On the basis of the reactivities of these MAb, it appears that although the Igh-Ce and Igh-Cd haplotypes are virtually identical at the Igh-3(gamma 2b), Igh-1(gamma 2a), and Igh-2(alpha) loci, they are highly divergent at the Igh-5(delta) and Igh-6(mu) loci. This indicates that the Igh-Cd haplotype may have resulted from a recombination between Igh-Ce and another haplotype.
用同种异型和H-2不相容的脾细胞免疫小鼠产生的杂交瘤，通过流式细胞术进行筛选。鉴定出针对IgD五种已知特异性中四种的单克隆抗体（MAb）。通过给定的单克隆抗体与经胰蛋白酶蛋白水解去除Fab片段的细胞结合的能力，确定这些特异性在IgD分子上的位置。Igh-5.1（存在于IgDa和IgDe上）和Igh-5.5（IgDe特有的）都位于Fab片段上。交叉阻断实验结果表明，除Igh-5.3外，识别相同特异性的单克隆抗体与相同的抗原决定簇结合。胰蛋白酶消化和阻断研究均表明，Igh-5.3由至少两个抗原决定簇组成。还鉴定出AF6-78.25，一种对b、d和n单倍型的IgM特异的单克隆抗体；该单克隆抗体定义了一种新的特异性，Igh-6.6。基于这些单克隆抗体的反应性，似乎尽管Igh-Ce和Igh-Cd单倍型在Igh-3（γ2b）、Igh-1（γ2a）和Igh-2（α）基因座上几乎相同，但它们在Igh-5（δ）和Igh-6（μ）基因座上高度不同。这表明Igh-Cd单倍型可能是Igh-Ce与另一种单倍型之间重组的结果。
