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一种被单克隆大鼠抗体识别的小鼠IgM同种异型决定簇（Igh-6.5）。

A mouse IgM allotypic determinant (Igh-6.5) recognized by a monoclonal rat antibody.

作者信息

Kung J T, Sharrow S O, Sieckmann D G, Lieberman R, Paul W E

出版信息

J Immunol. 1981 Sep;127(3):873-6.

PMID:6167630
Abstract

Two monoclonal rat anti-mouse IgM antibodies, Bet 1 and Bet 2, are described in this paper. Bet 1 defines a new allotypic determinant, Igh-6.5, expressed on IgM molecules in serum and on B lymphocytes, whereas Bet 2 recognizes a determinant on IgM molecules of all mouse strains tested. Both reagents bind to the IgM myeloma protein MOPC104E, but not to IgG myeloma proteins, including FLOPC21, MOPC21, and UPC10. Using serum from various mouse strains to inhibit the binding of Bet 1 to MOPC104E, 3 distinct inhibition patterns were found. BALB/c, DBA/2, and CBA sera inhibited strongly, C56BL/6 (B6), SJL, AKR, and NZB sera inhibited weakly, and A and AL sera showed no inhibition of binding of BET 1 to MOPC104E. All sera tested were equivalent in their inhibition of the binding of Bet 2 to MOPC104E. When spleen cells from different mouse strains were reacted with fluorescein-conjugated Bet 1 (F-Bet 1) and subjected to flow microfluorometry analysis, 3 types of staining patterns, corresponding to those obtained with the serum inhibition assay, were also found. The determinant recognized by Bet 1 is controlled by a gene linked to the Igh-C gene complex. C.AL20 behaved like AL, and C.B20 and BAB/14 behaved like B6, both in the serum inhibition assay and in flow microfluorometry analysis of spleen cells stained with F-Bet 1. In addition, the capacity of serum from individual (BALB/c X B6) X B6 backcross progeny to inhibit the binding of Bet 1 to MOPC104E was linked to the expression of the Ig-1a marker.

摘要

本文描述了两种大鼠抗小鼠IgM单克隆抗体，Bet 1和Bet 2。Bet 1定义了一种新的同种异型决定簇Igh-6.5，其在血清中的IgM分子和B淋巴细胞上表达，而Bet 2识别所有测试小鼠品系的IgM分子上的一个决定簇。两种试剂均与IgM骨髓瘤蛋白MOPC104E结合，但不与IgG骨髓瘤蛋白结合，包括FLOPC21、MOPC21和UPC10。使用来自各种小鼠品系的血清抑制Bet 1与MOPC104E的结合，发现了3种不同的抑制模式。BALB/c、DBA/2和CBA血清强烈抑制，C56BL/6（B6）、SJL、AKR和NZB血清抑制较弱，而A和AL血清未显示对BET 1与MOPC104E结合的抑制作用。所有测试血清在抑制Bet 2与MOPC104E结合方面等效。当来自不同小鼠品系的脾细胞与荧光素偶联的Bet 1（F-Bet 1）反应并进行流式微量荧光分析时，也发现了3种染色模式，与血清抑制试验获得的模式相对应。Bet 1识别的决定簇由与Igh-C基因复合体连锁的基因控制。在血清抑制试验和用F-Bet 1染色的脾细胞的流式微量荧光分析中，C.AL20的表现与AL相似，C.B20和BAB/14的表现与B6相似。此外，个体（BALB/c×B6）×B6回交后代血清抑制Bet 1与MOPC104E结合的能力与Ig-1a标记的表达相关。

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EMBO J. 1993 Apr;12(4):1555-60. doi: 10.1002/j.1460-2075.1993.tb05799.x.
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EMBO J. 1993 Jan;12(1):357-63. doi: 10.1002/j.1460-2075.1993.tb05664.x.
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J Ind Microbiol. 1995 May;14(5):371-6. doi: 10.1007/BF01569953.
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Frequency of B lymphocytes responsive to anti-immunoglobulin.对抗免疫球蛋白有反应的B淋巴细胞的频率
J Exp Med. 1982 May 1;155(5):1523-36. doi: 10.1084/jem.155.5.1523.
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J Exp Med. 1984 Dec 1;160(6):1605-21. doi: 10.1084/jem.160.6.1605.
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