Raucher H S, Hyatt A C, Barzilai A, Harris M B, Weiner M A, LeLeiko N S, Hodes D S
J Pediatr. 1984 Jan;104(1):29-33. doi: 10.1016/s0022-3476(84)80584-3.
We applied quantitative methods of analysis to all blood cultures drawn during the course of treatment in 28 children with Broviac catheters in a central vein. Thirty febrile episodes in 14 of these patients were evaluated. Samples of blood obtained from a peripheral vein and through the central catheter were cultured quantitatively on agar plates and nonquantitatively in standard broth media. Catheters were judged to be a source of septicemia nine times in seven children. In all nine positive catheter samples, the concentration of pathogens was 10 times as great as that observed in the peripheral venous sample. The blood drawn through the Broviac catheter contained greater than or equal to 2000 colony-forming units per milliliter in six cases. Quantitative cultures in two patients with septicemia not attributable to the catheter yielded low colony counts in the catheter sample. Cultures of blood samples drawn through the catheter when a child was well were not helpful in predicting subsequent septicemia. The technique of inoculating blood directly onto agar plates is easily performed and superior to standard broth cultures, because it detected pathogens within 16 hours and identified infections with multiple organisms.
我们对28例在中心静脉置有Broviac导管的儿童在治疗过程中采集的所有血培养标本应用了定量分析方法。对其中14例患者的30次发热发作进行了评估。从外周静脉和通过中心导管采集的血样在琼脂平板上进行定量培养，并在标准肉汤培养基中进行非定量培养。7名儿童的导管被判定为败血症的来源9次。在所有9份阳性导管样本中，病原体浓度是在外周静脉样本中观察到的10倍。通过Broviac导管抽取的血液在6例中每毫升含有大于或等于2000个菌落形成单位。在2例非导管所致败血症患者中，导管样本的定量培养菌落数较低。当儿童情况良好时，通过导管抽取的血样培养对预测随后的败血症没有帮助。将血液直接接种到琼脂平板上的技术操作简便，优于标准肉汤培养，因为它能在16小时内检测到病原体并识别多种微生物感染。
