Dessypris E N, Krantz S B
Br J Haematol. 1984 Feb;56(2):295-306. doi: 10.1111/j.1365-2141.1984.tb03957.x.
The effect of pure erythropoietin (EP) on human marrow day 15 burst-forming units-erythroid (BFU-E) was studied using a short-term liquid culture system containing 30% human serum. Non-adherent marrow cells were cultured in liquid medium for 0-48 h and then the number of BFU-E was assayed by the use of the plasma clot method. The addition of 1 U/ml of EP into the liquid culture medium resulted in maintenance of the number of BFU-E assayed after 24-48 h of incubation. The number of BFU-E recovered after 24-48 h culture was directly proportional to the concentration of EP present in the liquid medium. In addition, the proliferative status of BFU-E before and after exposure to EP was studied by 3H-thymidine and hydroxyurea suicide. It was found that EP doubles the percentage of BFU-E in DNA synthesis after 24-48 h of incubation in the liquid medium. This effect of EP on DNA synthesis by bone marrow day 15 BFU-E is detectable as early as 6 h after the onset of incubation and at EP concentrations as low as 0.2 U/ml of medium, a concentration present in the serum of moderately anaemic patients. The human marrow day 15 BFU-E is an EP-responsive cell and pure EP can induce it into DNA synthesis.
使用含30%人血清的短期液体培养系统，研究了纯红细胞生成素（EP）对人骨髓第15天红系爆式集落形成单位（BFU-E）的作用。将非贴壁骨髓细胞在液体培养基中培养0 - 48小时，然后采用血浆凝块法测定BFU-E的数量。向液体培养基中添加1 U/ml的EP可使孵育24 - 48小时后测定的BFU-E数量维持稳定。培养24 - 48小时后回收的BFU-E数量与液体培养基中EP的浓度成正比。此外，通过³H-胸腺嘧啶核苷和羟基脲自杀法研究了BFU-E暴露于EP前后的增殖状态。发现在液体培养基中孵育24 - 48小时后，EP使进行DNA合成的BFU-E百分比增加了一倍。EP对骨髓第15天BFU-E的DNA合成作用在孵育开始后6小时即可检测到，且在低至0.2 U/ml培养基的EP浓度下即可出现，这是中度贫血患者血清中的浓度。人骨髓第15天BFU-E是一种对EP有反应的细胞，纯EP可诱导其进行DNA合成。
