Yanokura M, Sawai Y, Tsukada K
Biochim Biophys Acta. 1984 Jan 24;797(1):94-8. doi: 10.1016/0304-4165(84)90386-6.
The activity of guanidoacetate methyltransferase has been measured in various tissues of mice and tumor cells. The creatine levels in tumor cells are high, although guanidoacetate methyltransferase activity was not detected. To confirm these results, labeled creatine was synthesized by guanidoacetate and its methyltransferase in the presence of S-adenosyl-L-[methyl-3H]methionine, and administered to a mouse with or without tumor cells. High levels of the uptake of the creatine were observed in tumor cells, intestine, heart and muscle of the mouse injected with [3H]creatine into a tumor-bearing mouse. These organs, however, had no detectable activity of guanidoacetate methyltransferase. Labeled creatine phosphate in the tissue was less than 10% compared to total labeled creatine.
已对小鼠的各种组织和肿瘤细胞中的胍基乙酸甲基转移酶活性进行了测定。尽管未检测到胍基乙酸甲基转移酶活性，但肿瘤细胞中的肌酸水平很高。为了证实这些结果，在S-腺苷-L-[甲基-³H]甲硫氨酸存在的情况下，由胍基乙酸及其甲基转移酶合成了标记的肌酸，并将其给予有或没有肿瘤细胞的小鼠。向荷瘤小鼠注射[³H]肌酸后，在该小鼠的肿瘤细胞、肠道、心脏和肌肉中观察到高水平的肌酸摄取。然而，这些器官中未检测到胍基乙酸甲基转移酶活性。与总标记肌酸相比，组织中标记的磷酸肌酸不到10%。
