Rodgers G M, Broze G J, Shuman M A
Blood. 1984 Feb;63(2):434-8.
Previously, we showed that cells derived from nonvascular tissues initiate clotting primarily by markedly increasing the activity of coagulation factor VII. Cells derived from vascular tissue do not normally exhibit this property (tissue factor activity). In this study, we have characterized the relationship between the tissue factor activity of cultured cells derived from normal tissues and the number of receptors they possess for coagulation factor VII. Only cultured nonvascular cells expressed tissue factor activity or possessed receptors for 125I-factor VII. Fetal lung cells, the nonvascular tissue with the largest amount of procoagulant and tissue factor activity, possessed the most receptors for 125I-factor VII (880,000/cell). Bovine corneal endothelial cells, the nonvascular tissue possessing the fewest number of receptors (2,400/cell), had the least amount of procoagulant or tissue factor activity. The affinity of nonvascular cells for 125I-factor VII varied for the cells studied (Kd congruent to 1.3-90 X 10(-10) M). Vascular cells expressed no tissue factor activity, nor did they bind 125I-factor VII. 125I-factor VII and unlabeled factor VII bound to cells had identical procoagulant activities. These results indicate that the ability of cultured cells to initiate coagulation may be regulated in part by the number of receptors they possess for factor VII.
此前，我们发现源自非血管组织的细胞主要通过显著提高凝血因子VII的活性来启动凝血过程。源自血管组织的细胞通常不表现出这种特性（组织因子活性）。在本研究中，我们已明确了源自正常组织的培养细胞的组织因子活性与其所拥有的凝血因子VII受体数量之间的关系。只有培养的非血管细胞表达组织因子活性或拥有125I-因子VII的受体。胎儿肺细胞作为具有最高促凝剂和组织因子活性的非血管组织，拥有最多的125I-因子VII受体（880,000个/细胞）。牛角膜内皮细胞作为拥有最少受体数量（2,400个/细胞）的非血管组织，其促凝剂或组织因子活性最低。所研究的非血管细胞对125I-因子VII的亲和力各不相同（解离常数约为1.3 - 90×10⁻¹⁰ M）。血管细胞既不表达组织因子活性，也不结合125I-因子VII。与细胞结合的125I-因子VII和未标记的因子VII具有相同的促凝活性。这些结果表明，培养细胞启动凝血的能力可能部分受其拥有的因子VII受体数量的调节。
