Rodgers G M, Greenberg C S, Shuman M A
Blood. 1983 Jun;61(6):1155-62.
The coagulant properties of intact bovine vascular cells (aortic endothelial and smooth muscle cells) and human vascular cells (cutaneous and foreskin microvascular cells, umbilical venous endothelium) grown in vitro were studied. Compared to nonvascular cells (fibroblasts, corneal endothelial cells, fetal lung or intestinal mucosal cells), vascular cells had little procoagulant activity. Radioimmunologic measurement of thrombin in recalcified plasma demonstrated markedly lower concentrations of thrombin in the presence of vascular endothelial and smooth muscle cells compared to corneal endothelial and fetal lung cells. The low thrombin concentrations were not a consequence of thrombin binding to the vascular cells nor were they due to accelerated thrombin inactivation by antithrombin-III or alpha 2-macroglobulin. Neither vascular cells nor the nonvascular cells promoted contact activation of plasma as measured by a sensitive specific assay for kallikrein. Studies with intact cell monolayers and purified factors VIIa and X indicated that while nonvascular cells express tissue factor activity, vascular cells do not exhibit this property. These data suggest that the nonthrombogenic nature of intact vascular cells is due to their failure to initiate contact activation and to express tissue factor activity. In addition, the primary difference in coagulant potential between vascular cells and nonvascular cells is the lack of tissue factor expression by the vascular cells.
研究了体外培养的完整牛血管细胞（主动脉内皮细胞和平滑肌细胞）和人血管细胞（皮肤和包皮微血管细胞、脐静脉内皮细胞）的凝血特性。与非血管细胞（成纤维细胞、角膜内皮细胞、胎儿肺或肠黏膜细胞）相比，血管细胞的促凝血活性较低。对重新钙化血浆中的凝血酶进行放射免疫测定表明，与角膜内皮细胞和胎儿肺细胞相比，在血管内皮细胞和平滑肌细胞存在的情况下，凝血酶浓度明显较低。低凝血酶浓度既不是凝血酶与血管细胞结合的结果，也不是抗凝血酶III或α2-巨球蛋白加速凝血酶失活的结果。通过一种灵敏的激肽释放酶特异性测定法测量，血管细胞和非血管细胞均未促进血浆的接触激活。对完整细胞单层以及纯化的因子VIIa和X的研究表明，虽然非血管细胞表达组织因子活性，但血管细胞不具有此特性。这些数据表明，完整血管细胞的抗血栓形成特性是由于它们未能引发接触激活以及未能表达组织因子活性。此外，血管细胞和非血管细胞在凝血潜能上的主要差异在于血管细胞缺乏组织因子表达。
