血浆中肉碱和酰基肉碱测定的误差来源

Sources of error in determinations of carnitine and acylcarnitine in plasma.

作者信息

Fishlock R C, Bieber L L, Snoswell A M

出版信息

Clin Chem. 1984 Feb;30(2):316-8.

Abstract

Radioactive and nonradioactive L-carnitine and acyl-L-carnitine were used to evaluate the washing procedures used during the determination of free, total, short-chain, and long-chain acylcarnitine in human and sheep plasma. The volume of fluid trapped by the protein precipitated by perchloric acid is approximately 24% of the total fluid volume and thus contains 24% of free carnitine and short-chain acylcarnitine. Washing twice with distilled water removes about 25% of the long-chain acylcarnitine along with the trapped free carnitine and short-chain acylcarnitines. Washing the pellet twice with a 60 g/L solution of perchloric acid completely removes the trapped free carnitine and short-chain acylcarnitine but does not remove the bound long-chain acylcarnitines. Thus washing with perchloric acid is essential for accurate measurement of long-chain acylcarnitines in plasma samples.

摘要

放射性和非放射性的左旋肉碱及酰基左旋肉碱被用于评估在测定人及绵羊血浆中游离、总、短链和长链酰基肉碱时所采用的洗涤程序。高氯酸沉淀的蛋白质截留的液体体积约占总液体体积的24%，因此含有24%的游离肉碱和短链酰基肉碱。用蒸馏水洗涤两次可去除约25%的长链酰基肉碱以及截留的游离肉碱和短链酰基肉碱。用60 g/L的高氯酸溶液洗涤沉淀两次可完全去除截留的游离肉碱和短链酰基肉碱，但不会去除结合的长链酰基肉碱。因此，用高氯酸洗涤对于准确测量血浆样本中的长链酰基肉碱至关重要。