Osheroff P L, Tahara S M, Chiang T R
Clin Immunol Immunopathol. 1984 Feb;30(2):188-96. doi: 10.1016/0090-1229(84)90053-9.
Recombinant human leukocyte B interferon (rIFN-alpha B) was purified to approximately 60% on a column of monoclonal antibody to IFN-alpha. This partially purified rIFN-alpha B was in turn used as immunogen in obtaining four monoclonal antibodies to this recombinant interferon. All four antibodies, while showing varying degrees of cross-reactivity to some other rIFN-alpha subtypes, showed specificity to monomeric and dimeric forms of rIFN-alpha B only and not to the contaminants in the partially purified preparation as demonstrated in the Western blot immunoassay.
重组人白细胞B干扰素（rIFN-αB）在抗IFN-α单克隆抗体柱上纯化至约60%。这种部分纯化的rIFN-αB又被用作免疫原，以获得针对这种重组干扰素的四种单克隆抗体。在蛋白质印迹免疫分析中表明，所有这四种抗体虽然对其他一些rIFN-α亚型表现出不同程度的交叉反应，但仅对rIFN-αB的单体和二聚体形式具有特异性，而对部分纯化制剂中的污染物没有特异性。
