Phillips A L, Gray J C
Eur J Biochem. 1984 Feb 1;138(3):591-5. doi: 10.1111/j.1432-1033.1984.tb07955.x.
Three components of the cytochrome b-f complex, cytochrome f, cytochrome b-563 and a 15.2-kDa polypeptide, were labelled with radioactive amino acids in isolated pea chloroplasts incubated in the light with added Mg-ATP. The assembly of cytochrome b-563 (19.5 kDa) into the cytochrome complex required the presence of added Mg-ATP whereas cytochrome f (37.3 kDa) and the 15.2-kDa polypeptide were assembled in its absence. Incorporation of [35S]methionine into the polypeptide chain of cytochrome b-563 and the 15.2-kDa component was confirmed by peptide mapping of the products of partial digestion with papain.
细胞色素b-f复合体的三个组分，即细胞色素f、细胞色素b-563和一个15.2 kDa的多肽，在添加了Mg-ATP的豌豆叶绿体中进行光照孵育时，用放射性氨基酸进行了标记。细胞色素b-563（19.5 kDa）装配到细胞色素复合体中需要添加Mg-ATP，而细胞色素f（37.3 kDa）和15.2 kDa的多肽在不添加Mg-ATP的情况下也能装配。用木瓜蛋白酶部分消化产物进行肽图谱分析，证实了[35S]甲硫氨酸掺入到细胞色素b-563和15.2 kDa组分的多肽链中。
