Highfield P E, Ellis R J
Biochim Biophys Acta. 1976 Sep 20;447(1):20-7. doi: 10.1016/0005-2787(76)90091-5.
Isolated intact pea chloroplasts use light energy to synthesise N-formayl [35S]-methionylpuromycin when incubated with L-[35S]methionine and puromycin. Control experiments establish that this synthesis occurs on chloroplast ribosomes, and not on contaminating mitochondrial or bacterial ribosomes. The amount of N-formylmethionylpuromycin formed suggests that each messenger RNA that is being translated in vitro undergoes initiation at least twice. We conclude that isolated, intact pea chloroplasts carry out the initiation of protein synthesis, as well as the elongation and termination of polypeptide chains.
当与L-[35S]甲硫氨酸和嘌呤霉素一起温育时，分离得到的完整豌豆叶绿体利用光能合成N-甲酰基[35S]-甲硫氨酰嘌呤霉素。对照实验证实这种合成发生在叶绿体核糖体上，而不是在污染的线粒体或细菌核糖体上。所形成的N-甲酰甲硫氨酰嘌呤霉素的量表明，每个在体外进行翻译的信使核糖核酸至少经历两次起始过程。我们得出结论，分离得到的完整豌豆叶绿体能够进行蛋白质合成的起始过程，以及多肽链的延伸和终止过程。
