培养的大鼠肌管中乙酰胆碱受体簇的组织是钙依赖性的。
Organization of acetylcholine receptor clusters in cultured rat myotubes is calcium dependent.
作者信息
Bursztajn S, McManaman J L, Appel S H
出版信息
J Cell Biol. 1984 Feb;98(2):507-17. doi: 10.1083/jcb.98.2.507.
Abstract
The effect of extracellular Ca2+ concentration and myasthenic globulin on the distribution and appearance of acetylcholine receptor (AChR) clusters on rat myotubes was studied with tetramethyl-rhodamine-labeled alpha BTX. Low Ca2+ medium (2.5 X 10(-5) M) caused a time-dependent loss of AChR clusters, and a concomitant increase in small punctate areas of fluorescence. High Ca2+ concentrations (1.5 X 10(-2) M) increased the size of AChR clusters without altering AChR synthesis. These changes were not observed with other divalent ions. In the presence of myasthenic globulin, the rate of AChR turnover increases, and AChR clusters are rapidly dispersed. High Ca2+ concentration partially protects the AChR clusters from dispersal and decreases the rate of receptor turnover.
摘要
用四甲基罗丹明标记的α-银环蛇毒素研究了细胞外Ca2+浓度和重症肌无力球蛋白对大鼠肌管上乙酰胆碱受体（AChR）簇分布和外观的影响。低钙培养基（2.5×10^(-5) M）导致AChR簇随时间丢失，并伴随荧光小斑点区域增加。高钙浓度（1.5×10^(-2) M）增加了AChR簇的大小，而不改变AChR的合成。其他二价离子未观察到这些变化。在重症肌无力球蛋白存在的情况下，AChR的周转速率增加，AChR簇迅速分散。高钙浓度部分保护AChR簇不分散，并降低受体周转速率。
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