Prives J, Fulton A B, Penman S, Daniels M P, Christian C N
J Cell Biol. 1982 Jan;92(1):231-6. doi: 10.1083/jcb.92.1.231.
To monitor the interaction of cell surface acetylcholine (AcCho) receptors with the cytoskeleton, cultured muscle cells were labeled with radioactive or fluorescent alpha-bungarotoxin and extracted with Triton X-100, using conditions that preserve internal structure. A significant population of the AcCho receptors is retained on the skeletal framework remaining after detergent extraction. The skeleton organization responsible for restricting AcCho receptors to a patched region may also result in their retention after detergent extraction.
为监测细胞表面乙酰胆碱(AcCho)受体与细胞骨架的相互作用,在保留内部结构的条件下,用放射性或荧光α-银环蛇毒素标记培养的肌肉细胞,并用Triton X-100进行提取。去污剂提取后,大量的AcCho受体保留在剩余的骨架结构上。负责将AcCho受体限制在一个斑块区域的骨架组织,也可能导致它们在去污剂提取后仍被保留。