培养的胚胎肌细胞表面细胞骨架框架与乙酰胆碱受体的相互作用。
Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture.
作者信息
Prives J, Fulton A B, Penman S, Daniels M P, Christian C N
出版信息
J Cell Biol. 1982 Jan;92(1):231-6. doi: 10.1083/jcb.92.1.231.
Abstract
To monitor the interaction of cell surface acetylcholine (AcCho) receptors with the cytoskeleton, cultured muscle cells were labeled with radioactive or fluorescent alpha-bungarotoxin and extracted with Triton X-100, using conditions that preserve internal structure. A significant population of the AcCho receptors is retained on the skeletal framework remaining after detergent extraction. The skeleton organization responsible for restricting AcCho receptors to a patched region may also result in their retention after detergent extraction.
摘要
为监测细胞表面乙酰胆碱（AcCho）受体与细胞骨架的相互作用，在保留内部结构的条件下，用放射性或荧光α-银环蛇毒素标记培养的肌肉细胞，并用Triton X-100进行提取。去污剂提取后，大量的AcCho受体保留在剩余的骨架结构上。负责将AcCho受体限制在一个斑块区域的骨架组织，也可能导致它们在去污剂提取后仍被保留。
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