培养的胚胎肌细胞表面细胞骨架框架与乙酰胆碱受体的相互作用。
Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture.
作者信息
Prives J, Fulton A B, Penman S, Daniels M P, Christian C N
出版信息
J Cell Biol. 1982 Jan;92(1):231-6. doi: 10.1083/jcb.92.1.231.
Abstract
To monitor the interaction of cell surface acetylcholine (AcCho) receptors with the cytoskeleton, cultured muscle cells were labeled with radioactive or fluorescent alpha-bungarotoxin and extracted with Triton X-100, using conditions that preserve internal structure. A significant population of the AcCho receptors is retained on the skeletal framework remaining after detergent extraction. The skeleton organization responsible for restricting AcCho receptors to a patched region may also result in their retention after detergent extraction.
摘要
为监测细胞表面乙酰胆碱（AcCho）受体与细胞骨架的相互作用，在保留内部结构的条件下，用放射性或荧光α-银环蛇毒素标记培养的肌肉细胞，并用Triton X-100进行提取。去污剂提取后，大量的AcCho受体保留在剩余的骨架结构上。负责将AcCho受体限制在一个斑块区域的骨架组织，也可能导致它们在去污剂提取后仍被保留。
相似文献
1
Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture.
J Cell Biol. 1982 Jan;92(1):231-6. doi: 10.1083/jcb.92.1.231.
2
Microtubules, microfilaments and the transport of acetylcholine receptors in embryonic myotubes.
Exp Cell Res. 1985 Aug;159(2):430-40. doi: 10.1016/s0014-4827(85)80016-1.
3
Role of the cytoskeleton in the formation, stabilization, and removal of acetylcholine receptor clusters in cultured muscle cells.
J Cell Biol. 1984 Jul;99(1 Pt 1):148-54. doi: 10.1083/jcb.99.1.148.
4
Membrane-cytoskeleton associations during myogenesis deviate from traditional definitions.
Exp Cell Res. 1989 Jul;183(1):1-23. doi: 10.1016/0014-4827(89)90414-x.
5
A factor from neurons increases the number of acetylcholine receptor aggregates on cultured muscle cells.
Proc Natl Acad Sci U S A. 1978 Aug;75(8):4011-5. doi: 10.1073/pnas.75.8.4011.
6
Microtubules and the formation of acetylcholine receptor clusters in chick embryonic muscle cells.
Eur J Cell Biol. 1985 Nov;39(1):173-8.
7
Tunicamycin increases desensitization of acetylcholine receptors in cultured mouse muscle cells.
Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1808-11. doi: 10.1073/pnas.88.5.1808.
8
Nonequivalence of alpha-bungarotoxin receptors and acetylcholine receptors in chick sympathetic neurons.
Proc Natl Acad Sci U S A. 1978 Feb;75(2):1016-20. doi: 10.1073/pnas.75.2.1016.
9
Newly synthesized acetylcholine receptors are located in the Golgi apparatus.
J Cell Biol. 1978 Jan;76(1):237-44. doi: 10.1083/jcb.76.1.237.
10
Proceedings: Appearance and disappearance of acetylcholine receptor during differentiation of embryonic chick skeletal muscle in vitro.
Isr J Med Sci. 1975 Nov;11(11):1192.
引用本文的文献
1
HDAC6 regulates microtubule stability and clustering of AChRs at neuromuscular junctions.
J Cell Biol. 2020 Aug 3;219(8). doi: 10.1083/jcb.201901099.
2
The formation of complex acetylcholine receptor clusters requires MuSK kinase activity and structural information from the MuSK extracellular domain.
Mol Cell Neurosci. 2012 Apr;49(4):475-86. doi: 10.1016/j.mcn.2011.12.007. Epub 2011 Dec 23.
3
The role of the cytoskeleton in neuromuscular junction formation.
J Mol Neurosci. 2006;30(1-2):115-8. doi: 10.1385/JMN:30:1:115.
4
Src-family kinases stabilize the neuromuscular synapse in vivo via protein interactions, phosphorylation, and cytoskeletal linkage of acetylcholine receptors.
J Neurosci. 2005 Nov 9;25(45):10479-93. doi: 10.1523/JNEUROSCI.2103-05.2005.
5
Src-class kinases act within the agrin/MuSK pathway to regulate acetylcholine receptor phosphorylation, cytoskeletal anchoring, and clustering.
J Neurosci. 2001 Jun 1;21(11):3806-18. doi: 10.1523/JNEUROSCI.21-11-03806.2001.
6
Agrin-induced phosphorylation of the acetylcholine receptor regulates cytoskeletal anchoring and clustering.
J Cell Biol. 2001 Apr 2;153(1):1-12. doi: 10.1083/jcb.153.1.1.
7
Agrin-induced acetylcholine receptor clustering is mediated by the small guanosine triphosphatases Rac and Cdc42.
J Cell Biol. 2000 Jul 10;150(1):205-12. doi: 10.1083/jcb.150.1.205.
8
Role of actin in anchoring postsynaptic receptors in cultured hippocampal neurons: differential attachment of NMDA versus AMPA receptors.
J Neurosci. 1998 Apr 1;18(7):2423-36. doi: 10.1523/JNEUROSCI.18-07-02423.1998.
9
Effects of antimitotic agents on secretion and detergent extractibility of adrenal nicotinic acetylcholine receptors.
Cell Mol Neurobiol. 1997 Oct;17(5):447-54. doi: 10.1023/a:1026350619823.
10
Spatial and temporal dissection of immediate and early events following cadherin-mediated epithelial cell adhesion.
J Cell Biol. 1993 Mar;120(5):1217-26. doi: 10.1083/jcb.120.5.1217.
本文引用的文献
1
Ultrastructural characterization of surface specializations containing high-density acetylcholine receptors on embryonic chick myotubes in vivo and in vitro.
Dev Biol. 1981 Jul 30;85(2):267-86. doi: 10.1016/0012-1606(81)90259-1.
2
A factor from neurons induces partial immobilization of nonclustered acetylcholine receptors on cultured muscle cells.
J Cell Biol. 1981 Feb;88(2):459-62. doi: 10.1083/jcb.88.2.459.
3
Developmental reorganization of the skeletal framework and its surface lamina in fusing muscle cells.
J Cell Biol. 1981 Oct;91(1):103-12. doi: 10.1083/jcb.91.1.103.
4
The localization of acetylcholine receptor clusters in areas of cell-substrate contact in cultures of rat myotubes.
Cell. 1980 Aug;21(1):25-35. doi: 10.1016/0092-8674(80)90111-7.
5
Acetylcholine receptors. Distribution and extrajunctional density in rat diaphragm after denervation correlated with acetylcholine sensitivity.
J Gen Physiol. 1972 Sep;60(3):248-62. doi: 10.1085/jgp.60.3.248.
6
Cholinergic receptor molecules and cholinesterase molecules at mouse skeletal muscle junctions.
Nature. 1971 Nov 26;234(5326):207-9. doi: 10.1038/234207a0.
7
Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents.
J Supramol Struct. 1973;1(3):233-48. doi: 10.1002/jss.400010308.
8
The distribution of acetylcholine sensitivity over uninnervated and innervated muscle fibers grown in cell culture.
Dev Biol. 1973 Mar;31(1):147-62. doi: 10.1016/0012-1606(73)90326-6.
9
Appearance of acetylcholine receptor in differentiating cultures of embryonic chick breast muscle.
J Cell Biol. 1973 Oct;59(1):241-5. doi: 10.1083/jcb.59.1.241.
10
Acetycholine receptor production and incorporation into membranes of developing muscle fibers.
Dev Biol. 1973 Jan;30(1):153-65. doi: 10.1016/0012-1606(73)90054-7.
Zotero 插件