de Blas A L
J Neurosci. 1984 Jan;4(1):265-73. doi: 10.1523/JNEUROSCI.04-01-00265.1984.
The cytoarchitecture of the cerebellar Bergmann fibers in the adult rat was investigated. Two monoclonal antibodies, one specific for the Bergmann fibers and astrocyte processes and the other specific for the cell bodies and dendrites of the Purkinje cells as well as an antiserum to the glial fibrillary acidic protein, were used in immunocytochemical peroxidase-antiperoxidase assays. The Bergmann fibers are revealed as columns organized in long vertical palisades parallel to the longitudinal plane of the folium. The palisades are not continuous; instead they are formed by sets of two to six aligned Bergmann fibers. Each of these sets of Bergmann fibers is separated from its longitudinally aligned neighbors by gaps. Each Bergmann fiber is formed by a bundle of two to four Bergmann glia processes which frequently show a helical organization. These results help to reconcile the different views on the organization of the Bergmann fibers derived from the studies done with the light microscope versus those done with the electron microscope. The Bergmann glia may play a fundamental role in directing the geometrical organization of the cerebellar constituents.
对成年大鼠小脑伯格曼纤维的细胞构筑进行了研究。在免疫细胞化学过氧化物酶-抗过氧化物酶试验中，使用了两种单克隆抗体，一种对伯格曼纤维和星形胶质细胞突起具有特异性，另一种对浦肯野细胞的胞体和树突具有特异性，以及一种针对胶质纤维酸性蛋白的抗血清。伯格曼纤维呈现为与小叶纵平面平行的长垂直栅栏状排列的柱状结构。这些栅栏并不连续；相反，它们由两到六根排列整齐的伯格曼纤维组成。每组这样的伯格曼纤维与其纵向排列的相邻纤维之间由间隙隔开。每根伯格曼纤维由两到四根伯格曼胶质细胞突起束形成，这些突起经常呈现螺旋状排列。这些结果有助于调和通过光学显微镜和电子显微镜研究得出的关于伯格曼纤维组织结构的不同观点。伯格曼胶质细胞可能在指导小脑成分的几何组织方面发挥着重要作用。
