Johnson W T, Evans G W
J Nutr. 1984 Jan;114(1):180-90. doi: 10.1093/jn/114.1.180.
Zinc, iron and copper concentrations were measured in several organs from streptozotocin-diabetic and normal male, Long-Evans rats that were maintained for 21 days on a dietary regimen designed to study the combined or singular effects of insulin-dependent diabetes, dietary protein and dietary minerals on the tissue content of trace metals. The diets contained either 20 ppm zinc and iron, 5 ppm copper and 20% protein (HMHP); 8 ppm zinc and iron, 2 ppm copper and 8.3% protein (LMLP); 20 ppm zinc and iron, 5 ppm copper and 8.3% protein (HMLP) or 8 ppm zinc and iron, 2 ppm copper and 20% protein (LMHP). The concentrations of zinc, iron and copper in liver, zinc and iron in kidney and iron in femur were elevated in the diabetic rats and were not influenced by dietary protein and mineral interaction. However, dietary protein, mineral or protein X mineral interaction significantly affected trace metal concentrations of several organs in diabetic rats but had no significant effect in normal rats. Specifically, copper concentration in kidney and duodenum of diabetic rats were influenced by protein X mineral interaction, duodenal zinc concentrations were higher in diabetic rats fed high mineral diets (HMHP and HMLP) compared to diabetic rats fed low mineral diets (LMHP and LMLP) and femur zinc concentration was higher in diabetic rats fed high protein diets (HMHP and LMLP) compared to diabetic rats fed low protein diets (HMLP and LMLP). While hepatic picolinic carboxylase was elevated severalfold in diabetic rats, it was highest in the diabetic rats fed high protein diets (HPHM and HPLM) suggesting that picolinic acid may, at least in part, mediate the effects of dietary protein and minerals on tissue trace metal concentrations in diabetic rats.
在链脲佐菌素诱导的糖尿病雄性Long-Evans大鼠和正常雄性大鼠的多个器官中测量了锌、铁和铜的浓度。这些大鼠按照旨在研究胰岛素依赖型糖尿病、膳食蛋白质和膳食矿物质对痕量金属组织含量的联合或单一作用的饮食方案维持21天。饮食中含有20 ppm锌和铁、5 ppm铜以及20%蛋白质（HMHP）；8 ppm锌和铁、2 ppm铜以及8.3%蛋白质（LMLP）；20 ppm锌和铁、5 ppm铜以及8.3%蛋白质（HMLP）或8 ppm锌和铁、2 ppm铜以及20%蛋白质（LMHP）。糖尿病大鼠肝脏中的锌、铁和铜浓度，肾脏中的锌和铁浓度以及股骨中的铁浓度升高，且不受膳食蛋白质和矿物质相互作用的影响。然而，膳食蛋白质、矿物质或蛋白质X矿物质相互作用显著影响糖尿病大鼠多个器官的痕量金属浓度，但对正常大鼠无显著影响。具体而言，糖尿病大鼠肾脏和十二指肠中的铜浓度受蛋白质X矿物质相互作用影响，与喂食低矿物质饮食（LMHP和LMLP）的糖尿病大鼠相比，喂食高矿物质饮食（HMHP和HMLP）的糖尿病大鼠十二指肠锌浓度更高，与喂食低蛋白质饮食（HMLP和LMLP）的糖尿病大鼠相比，喂食高蛋白质饮食（HMHP和LMLP）的糖尿病大鼠股骨锌浓度更高。虽然糖尿病大鼠肝脏中的吡啶甲酸羧化酶升高了几倍，但在喂食高蛋白质饮食（HPHM和HPLM）的糖尿病大鼠中最高，这表明吡啶甲酸可能至少部分介导了膳食蛋白质和矿物质对糖尿病大鼠组织痕量金属浓度的影响。
