Rafael J, Patzelt J, Schäfer H, Elmadfa I
J Nutr. 1984 Feb;114(2):255-62. doi: 10.1093/jn/114.2.255.
Rats were fed a diet poor (0.05%) in essential fatty acids (EFA) with hydrogenated coconut oil as fat component, or a control diet containing 4% of the total energy intake in the form of linoleic acid. Effects of dietary EFA deficiency were investigated during a period of 2-30 weeks. Growth retardation becomes significant after 4 weeks of deficiency and attains about 25% when the deficiency is maintained for longer than 12 weeks. Respiration, body weight and age of EFA-deficient rats and controls are in a nonlinear relationship. Basal respiration in relation to the body weight is significantly increased by EFA deficiency; it is unchanged when related to total animals under the employed experimental conditions. Oxidative phosphorylation in isolated liver mitochondria is unaffected by EFA deficiency, i.e., the increased metabolic rate of EFA-deficient rats, related to the body weight, cannot be explained from impaired functional integrity of the inner mitochondrial membrane. Respiratory chain enzyme activities in mitochondria from heart and skeletal muscle and specific amounts of mitochondria in these tissues are unchanged by EFA deficiency.
给大鼠喂食以氢化椰子油为脂肪成分的必需脂肪酸（EFA）含量低（0.05%）的饮食，或喂食一种以亚油酸形式提供总能量摄入4%的对照饮食。在2至30周的时间段内研究饮食中EFA缺乏的影响。缺乏4周后生长迟缓变得显著，当缺乏持续超过12周时，生长迟缓达到约25%。EFA缺乏的大鼠和对照大鼠的呼吸、体重和年龄呈非线性关系。EFA缺乏使基础呼吸相对于体重显著增加；在所用实验条件下，相对于动物总数，基础呼吸没有变化。EFA缺乏不影响分离的肝线粒体中的氧化磷酸化，即，EFA缺乏的大鼠相对于体重增加的代谢率不能用线粒体内膜功能完整性受损来解释。EFA缺乏不会改变心脏和骨骼肌线粒体中的呼吸链酶活性以及这些组织中线粒体的特定数量。
