Lin L S, Caritis S N, Wong L K
J Pharm Sci. 1984 Jan;73(1):131-3. doi: 10.1002/jps.2600730137.
A sensitive and specific assay for ritodrine in serum was developed using high-performance liquid chromatography (HPLC) with electrochemical detection. Serum samples were alkalinized to pH 9.4 by the addition of a sodium carbonate buffer and extracted with ethyl acetate. The extracts were evaporated to dryness and the residues were reconstituted in the HPLC mobile phase and chromatographed on a octadecylsilane reverse-phase column. The detection of ritodrine was achieved by an electrochemical detector with a glassy carbon electrode. The sensitivity was 0.2 ng for on-column injection. The extraction efficiency was 80%.
采用高效液相色谱（HPLC）结合电化学检测技术，开发了一种灵敏且特异的血清中利托君检测方法。通过添加碳酸钠缓冲液将血清样品碱化至pH 9.4，并用乙酸乙酯萃取。萃取液蒸发至干，残渣用HPLC流动相复溶后，在十八烷基硅烷反相柱上进行色谱分析。利托君的检测通过带有玻璃碳电极的电化学检测器完成。柱上进样的灵敏度为0.2 ng。萃取效率为80%。
