Chu S Y
J Pharm Sci. 1978 Nov;67(11):1623-5. doi: 10.1002/jps.2600671131.
Assay of 8-hydroxycarteolol (a metabolite of carteolol) was achieved using high-pressure liquid chromatography with electrochemical detection. Plasma or urine samples alkalinized by addition of a sodium carbonate solution were extracted with ethyl acetate or chloroform. The residues from evaporation of the organic extracts were redissolved in pH 2.1 phosphate buffer, and the solutions were chromatographed on a Partisil 10 SCX chromatographic column. The detection of 8-hydroxycarteolol was accomplished using an electrochemical detector. The procedure is rapid, specific, and highly sensitive. Reproducible results can be obtained, with relative standard deviations from analysis of replicate samples within +/- 8%. With 1-ml samples, the lower quantifiable concentrations of 8-hydroxycarteolol in plasma and urine are approximately 5 and 25 ng/ml, respectively.
使用带电化学检测的高压液相色谱法对8-羟基卡替洛尔（卡替洛尔的一种代谢物）进行测定。通过加入碳酸钠溶液碱化的血浆或尿液样本用乙酸乙酯或氯仿萃取。有机萃取液蒸发后的残留物重新溶解于pH 2.1的磷酸盐缓冲液中，溶液在Partisil 10 SCX色谱柱上进行色谱分析。使用电化学检测器完成对8-羟基卡替洛尔的检测。该方法快速、特异且高度灵敏。可获得可重现的结果，重复样本分析的相对标准偏差在±8%以内。对于1毫升样本，血浆和尿液中8-羟基卡替洛尔的最低可定量浓度分别约为5纳克/毫升和25纳克/毫升。
