Summerfield J A, Applebaum-Bowden D, Hazzard W R
Proc Soc Exp Biol Med. 1984 Feb;175(2):158-63. doi: 10.3181/00379727-175-41781.
Plasma clearance of triglyceride-rich lipoproteins appears decreased in aged humans and rats and may be due to lowered activities of the lipases responsible for lipid degradation. This study was designed to examine differential effects of age and diet on lipoprotein lipase (LPL) activity of adipose and heart tissue and hepatic triglyceride lipase (HTGL) activity. LPL and HTGL activities were examined in 3- and 13-month-old Sprague-Dawley rats after they had consumed either a high-carbohydrate or a high-fat diet for 14 days. The data were analyzed for age and diet differences by two-way analysis of variance. Although animals in the two age groups consumed diets of equal caloric content, the older rats gained less weight. Rats on the high-carbohydrate diet consumed less calories and gained less weight than the fat fed rats in both age groups. Neither heart nor adipose tissue LPL activity differed when examined for age or diet. HTGL activity levels, while not affected by age, were higher in the carbohydrate fed rats (P = 0.014). Regardless of age group, fasting plasma cholesterol levels were significantly higher in the carbohydrate-fed rats than fat-fed rats (P = 0.002). Thus, the diet effect was much stronger than the age effect for HTGL and plasma cholesterol levels.
富含甘油三酯的脂蛋白的血浆清除率在老年人类和大鼠中似乎有所下降，这可能是由于负责脂质降解的脂肪酶活性降低所致。本研究旨在探讨年龄和饮食对脂肪组织和心脏组织的脂蛋白脂肪酶（LPL）活性以及肝脏甘油三酯脂肪酶（HTGL）活性的不同影响。在3个月和13个月大的Sprague-Dawley大鼠分别食用高碳水化合物或高脂肪饮食14天后，检测其LPL和HTGL活性。通过双向方差分析对数据进行年龄和饮食差异分析。尽管两个年龄组的动物摄入的饮食热量相同，但老年大鼠体重增加较少。在两个年龄组中，高碳水化合物饮食组的大鼠比高脂肪饮食组的大鼠摄入的热量更少，体重增加也更少。在按年龄或饮食进行检测时，心脏和脂肪组织的LPL活性均无差异。HTGL活性水平虽不受年龄影响，但在高碳水化合物饮食组的大鼠中更高（P = 0.014）。无论年龄组如何，高碳水化合物饮食组大鼠的空腹血浆胆固醇水平均显著高于高脂肪饮食组大鼠（P = 0.002）。因此，对于HTGL和血浆胆固醇水平而言，饮食效应比年龄效应更强。
