Burlingame G A, McElhaney J, Bennett M, Pipes W O
Appl Environ Microbiol. 1984 Jan;47(1):56-60. doi: 10.1128/aem.47.1.56-60.1984.
The membrane filter (MF) method for detection and enumeration of coliform bacteria in drinking water requires that the coliforms both grow and produce a green metallic sheen when the filter is incubated on modified Endo medium at 35 degrees C for 22 h. Large numbers of noncoliform bacteria, which are enumerated by the standard plate count (SPC) technique, can interfere with the detection of coliforms on MF. This paper presents quantitative evidence from laboratory experiments on the interference of specific SPC bacteria on coliform colony sheen production on MF. Pseudomonas aeruginosa and Aeromonas hydrophila caused significant reductions in Escherichia coli sheen colony counts when present at 3,000 and 220 per filter, respectively. The Flavobacterium sp. and Bacillus sp. selected for this study from SPC did not interfere with coliform colony sheen production. Excessive crowding of E. coli and Enterobacter cloacae colonies on MF also caused a reduction in the number of colonies that produced sheen. Even when there was no crowding (14 colonies per filter), only a fraction of the E. cloacae colonies produced sheen colonies on modified Endo medium.
用于检测和计数饮用水中大肠菌群的膜过滤（MF）法要求，当滤膜在改良的远藤培养基上于35摄氏度培养22小时时，大肠菌群既要生长又要产生绿色金属光泽。通过标准平板计数（SPC）技术计数的大量非大肠菌群细菌会干扰MF法对大肠菌群的检测。本文提供了实验室实验的定量证据，证明特定的SPC细菌对MF法中大肠菌群菌落光泽产生的干扰。当每片滤膜上分别存在3000个铜绿假单胞菌和220个嗜水气单胞菌时，会导致大肠杆菌光泽菌落计数显著减少。从SPC中选出用于本研究的黄杆菌属和芽孢杆菌属不会干扰大肠菌群菌落光泽的产生。MF上大肠杆菌和阴沟肠杆菌菌落过度密集也会导致产生光泽的菌落数量减少。即使没有菌落密集情况（每片滤膜14个菌落），在改良远藤培养基上也只有一部分阴沟肠杆菌菌落产生光泽菌落。
