细胞外基质的16S乙酰胆碱酯酶在培养的小鼠肌肉细胞内组装。
16S acetylcholinesterase of the extracellular matrix is assembled within mouse muscle cells in culture.
作者信息
Inestrosa N C
出版信息
Biochem J. 1984 Jan 15;217(2):377-81. doi: 10.1042/bj2170377.
Abstract
The present paper examines where the extracellular-matrix (ECM) 16S acetylcholinesterase (AChE, EC 3.1.1.7) is assembled in muscle cells in culture. The existence of an internal pool of 16S AChE was detected by using AChE inhibitors of differing membrane permeability. After irreversible inhibition of all cellular esterase, the newly synthesized 16S form appears in an intracellular compartment and is only later detected on the cell surface. Results show that the ECM 16S AChE is assembled within muscle cells.
摘要
本文研究了细胞外基质（ECM）16S 乙酰胆碱酯酶（AChE，EC 3.1.1.7）在培养的肌肉细胞中的组装位置。通过使用具有不同膜通透性的 AChE 抑制剂检测到了 16S AChE 内部池的存在。在不可逆地抑制所有细胞酯酶后，新合成的 16S 形式出现在细胞内区室中，随后才在细胞表面被检测到。结果表明，ECM 16S AChE 在肌肉细胞内组装。
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本文引用的文献
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A new and rapid colorimetric determination of acetylcholinesterase activity.一种新的快速比色法测定乙酰胆碱酯酶活性。
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