Manning T A, Wilson J E
Biochem Biophys Res Commun. 1984 Jan 13;118(1):90-6. doi: 10.1016/0006-291x(84)91071-4.
P1-(adenosine-5')-P3-(glucose-6)-triphosphate (Ap3glucose) is a linear uncompetitive inhibitor vs glucose and a linear mixed inhibitor vs ATP of brain hexokinase, an inhibition pattern inconsistent with binding of Ap3glucose to the catalytic site when either the rapid equilibrium random or ordered sequential mechanism, which have been proposed for this enzyme, is considered. It is concluded that inhibition results from binding to a discrete regulatory site. The apparent ability of the regulatory site to accommodate both hexose and nucleotide moieties is consistent with suggestions by previous investigators that the regulatory site on mammalian hexokinases may have evolved from what was originally a catalytic site.
P1-（腺苷-5'）-P3-（葡萄糖-6）-三磷酸（Ap3葡萄糖）对脑己糖激酶而言，对葡萄糖是线性非竞争性抑制剂，对ATP是线性混合型抑制剂，当考虑该酶已被提出的快速平衡随机或有序顺序机制时，这种抑制模式与Ap3葡萄糖结合到催化位点不一致。得出的结论是，抑制作用是由于与一个离散的调节位点结合所致。调节位点容纳己糖和核苷酸部分的明显能力与先前研究者的建议一致，即哺乳动物己糖激酶上的调节位点可能是从最初的催化位点进化而来的。
