Hansson G C, Karlsson K A, Thurin J
Biochim Biophys Acta. 1984 Mar 7;792(3):281-92. doi: 10.1016/0005-2760(84)90195-4.
The epithelial cells and the non-epithelial residue from large intestine of two inbred rat strains were separated and the glycosphingolipids characterized in comparison with earlier detailed data from small intestine of the same strains. Total acid and non-acid glycolipids were prepared and the non-acid glycolipids were further fractionated into subgroups as acetylated derivatives on silicic acid. The fractions obtained were characterized mainly by thin-layer chromatography, including binding of monoclonal anti-A and anti-B antibody to the chromatogram, and by direct-inlet mass spectrometry after derivatization. This combined technology allowed an overall conclusion from a small number of animals concerning relative amounts of glycolipids, microheterogeneity of blood group glycolipids and carbohydrate sequence and lipophilic components of major species of each subfraction. As for the small intestine, the two separated compartments differed distinctly in composition, with blood group fucolipids being confined to the epithelial cells, and a series of glycolipids with probably internal Gal alpha being restricted to the non-epithelial part. The main difference between large and small intestine concerned fucolipids of the epithelium. Three blood group B active glycolipids with four, six and seven sugars were detected which were absent from the small intestine. The four-sugar glycolipid was a major glycolipid with the structure Gal alpha 1----3Gal(2----1 alpha Fuc) beta 1----4Glc beta 1----1Cer. as reported before. The six-sugar glycolipid was shown by mass spectrometry and NMR spectroscopy to have the probable structure Gal alpha 1----3Gal(2----1 alpha Fuc)beta 1----3GlcNAc beta 1----3Gal beta 1----4Glc beta 1----1Cer. The seven-sugar glycolipid had an additional fucose linked to N-acetylhexosamine, as shown by mass spectrometry. Three blood group A active glycolipids with four, six and seven sugars were found in both rat strains, with sequences analogous to the B glycolipids but with a terminal GalNAc instead of Gal. The four- and six-sugar blood group A compounds, but not the seven-sugar glycolipid, have been found before in the small intestine of one of the rat strains. In the small intestine, on the other hand, a branched-chain twelve-sugar blood group A active glycolipid has been found which was absent from the large intestine. Therefore large intestine of both rat strains expressed glycolipid-based blood group A and B activity, while small intestine lacked B activity and showed A activity only in one of the strains.(ABSTRACT TRUNCATED AT 400 WORDS)
分离了两个近交系大鼠品系大肠的上皮细胞和非上皮残余物，并对糖鞘脂进行了表征，同时与同一品系小肠的早期详细数据进行了比较。制备了总酸性和非酸性糖脂，并将非酸性糖脂进一步分离成亚组，作为硅酸上的乙酰化衍生物。所获得的馏分主要通过薄层色谱法进行表征，包括单克隆抗A和抗B抗体与色谱图的结合，以及衍生化后的直接进样质谱法。这种联合技术使得能够从少量动物中得出关于糖脂相对含量、血型糖脂的微异质性、碳水化合物序列以及每个亚组分主要种类的亲脂成分的总体结论。与小肠一样，两个分离的部分在组成上有明显差异，血型岩藻糖脂局限于上皮细胞，而一系列可能含有内部Galα的糖脂则局限于非上皮部分。大肠和小肠之间的主要差异在于上皮细胞的岩藻糖脂。检测到三种具有四个、六个和七个糖的血型B活性糖脂，这些糖脂在小肠中不存在。四糖糖脂是一种主要的糖脂，结构为Galα1----3Gal(2----1αFuc)β1----4Glcβ1----1Cer，如先前报道的那样。通过质谱和核磁共振光谱显示，六糖糖脂可能具有结构Galα1----3Gal(2----1αFuc)β1----3GlcNAcβ1----3Galβ1----4Glcβ1----1Cer。通过质谱显示，七糖糖脂在N-乙酰己糖胺上连接了一个额外的岩藻糖。在两个大鼠品系中都发现了三种具有四个、六个和七个糖的血型A活性糖脂，其序列与B糖脂类似，但末端为GalNAc而非Gal。四糖和六糖血型A化合物，但不是七糖糖脂，之前在其中一个大鼠品系的小肠中被发现。另一方面，在小肠中发现了一种分支链的十二糖血型A活性糖脂，而在大肠中不存在。因此，两个大鼠品系大肠都表达基于糖脂的血型A和B活性，而小肠缺乏B活性，仅在其中一个品系中显示A活性。（摘要截断于400字）
