Segerson E C, Libby D W
Biol Reprod. 1984 Feb;30(1):126-33. doi: 10.1095/biolreprod30.1.126.
Lymphocytes harvested from the jugular vein (JV), uterine vein (UV) and uterine lumen (UL) of Day 14 (n = 7), estrous (n = 6) and ovariectomized (OVX, n = 5) nulliparous Dorset ewes were tested in vitro with two dosages (0.08 and 0.16 micrograms) of the mitogen, phytohemagglutinin (PHA). UL lymphocytes were aspirated from the uterine lumen of each horn following gentle curetting of the endometrium. Lymphocytes (1 X 10(5)/culture well) from each source and reproductive category were cultured in RPMI-1640 (with additives) and treated with 0 (control), 0.08 and 0.16 micrograms of PHA. At 48 h of culture, 0.1 microCi of tritiated [3H]thymidine was added and were harvested at 60 +/- 1 h of culture. A stimulation index (SI) was calculated for cultures containing PHA. Analysis of variance revealed that SI values for Day 14 and estrous ewes were affected (P less than 0.002) by lymphocyte source at each PHA dosage. For Day 14 ewes, mean SI values for JV lymphocytes were greater (P less than 0.05) than for UL lymphocytes at 0.08 (7.2 and 1.8, respectively) and 0.16 micrograms (8.7 and 2.5, respectively) of PHA. For estrous ewes, mean SI values for JV lymphocytes were greater (P less than 0.05) than for UL lymphocytes at 0.08 (5.7 and 1.4, respectively) and 0.16 micrograms (6.7 and 2.2, respectively) of PHA. Mean SI values for UV lymphocytes for Day 14 and estrous ewes were intermediate between mean SI values for JV and UL lymphocytes. For OVX ewes, SI values were not affected (P greater than 0.25) by lymphocyte source at either PHA dosage. Further, mean SI values for UL lymphocytes from OVX ewes were greater than mean SI values for UL lymphocytes from Day 14 and estrous ewes at 0.08 (P less than 0.05) and 0.16 micrograms (P less than 0.02) of PHA. For intact ewes, data indicated that UL PHA-treated lymphocytes were suppressed, and that the immunosuppressive factor that can influence lymphocytes associated with the uterine environment may be a pertinent immunological adjustment that is requisite for maintenance of the ovine conceptus allograft.
从14日龄（n = 7）、发情期（n = 6）和去卵巢（OVX，n = 5）的未生育多塞特母羊的颈静脉（JV）、子宫静脉（UV）和子宫腔（UL）采集淋巴细胞，用两种剂量（0.08和0.16微克）的促有丝分裂原植物血凝素（PHA）进行体外测试。在轻轻刮除子宫内膜后，从每个子宫角的子宫腔中吸出UL淋巴细胞。将来自每个来源和生殖类别的淋巴细胞（1×10⁵/培养孔）培养在含有添加剂的RPMI - 1640中，并用0（对照）、0.08和0.16微克的PHA处理。培养48小时后，加入0.1微居里的氚标记[³H]胸腺嘧啶核苷，并在培养60±1小时时收获。计算含有PHA的培养物的刺激指数（SI）。方差分析显示，在每个PHA剂量下，14日龄和发情期母羊的SI值受淋巴细胞来源的影响（P＜0.002）。对于14日龄母羊，在0.08（分别为7.2和1.8）和0.16微克（分别为8.7和2.5）的PHA剂量下，JV淋巴细胞的平均SI值高于UL淋巴细胞（P＜0.05）。对于发情期母羊，在0.08（分别为5.7和1.4）和0.16微克（分别为6.7和2.2）的PHA剂量下，JV淋巴细胞的平均SI值高于UL淋巴细胞（P＜0.05）。14日龄和发情期母羊的UV淋巴细胞的平均SI值介于JV和UL淋巴细胞的平均SI值之间。对于OVX母羊，在任何一种PHA剂量下，SI值均不受淋巴细胞来源的影响（P＞0.25）。此外，在0.08（P＜0.05）和0.16微克（P＜0.02）的PHA剂量下，OVX母羊的UL淋巴细胞的平均SI值高于14日龄和发情期母羊的UL淋巴细胞的平均SI值。对于未去势母羊，数据表明经PHA处理的UL淋巴细胞受到抑制，并且能够影响与子宫环境相关淋巴细胞的免疫抑制因子可能是维持绵羊同种异体胚胎所需的一种相关免疫调节。
