Chilton B S, Key T L
Biol Reprod. 1984 Feb;30(1):231-8. doi: 10.1095/biolreprod30.1.231.
The temporal effects of a single injection of estradiol (5 micrograms in corn oil) were studied in 18- to 23-week ovariectomized rabbits for up to 48 h. Changes in cytosol and nuclear progesterone receptor concentrations in the epithelial-stromal complex were correlated with ultrastructural evidence of glycoprotein synthesis by nonciliated epithelial cells. After exposure to estradiol for 12 h, the Golgi cisternae of a few of the nonciliated cells contained secretory granules. After 24 h exposure, the number of nonciliated cells containing secretory granules was increased as was the number of granules per cell. After 36 h exposure, both the intracellular complement of secretory granules and the concentration of cytosol progesterone receptor (15.1 +/- 2.7 pmol/mg DNA) had reached a maximum. The concentration of cytosol progesterone receptor had increased 2-fold over the concentration (7.8 +/- 0.8 pmol/mg DNA) for ovariectomized rabbits. The concentration of nuclear progesterone receptor had increased from 66.3 +/- 26.3 fmol/mg DNA to 1459.5 +/- 99.3 fmol/mg DNA. After 48 h exposure the cellular complement of secretory granules was reduced as was the concentration of cytosol (5.0 +/- 0.5 pmol/mg DNA) and nuclear (740.0 +/- 364.9 fmol/mg DNA) progesterone receptor. It is apparent that the production of secretory granules and progesterone receptor concentration are temporally dependent upon estradiol.
对18至23周龄卵巢切除的兔子单次注射雌二醇（5微克溶于玉米油）后的时间效应进行了长达48小时的研究。上皮-基质复合物中细胞质和细胞核孕酮受体浓度的变化与非纤毛上皮细胞糖蛋白合成的超微结构证据相关。暴露于雌二醇12小时后，一些非纤毛细胞的高尔基池含有分泌颗粒。暴露24小时后，含有分泌颗粒的非纤毛细胞数量增加，每个细胞中的颗粒数量也增加。暴露36小时后，分泌颗粒的细胞内含量和细胞质孕酮受体浓度（15.1±2.7 pmol/mg DNA）均达到最大值。细胞质孕酮受体浓度比卵巢切除兔子的浓度（7.8±0.8 pmol/mg DNA）增加了2倍。细胞核孕酮受体浓度从66.3±26.3 fmol/mg DNA增加到1459.5±99.3 fmol/mg DNA。暴露48小时后，分泌颗粒的细胞含量减少，细胞质（5.0±0.5 pmol/mg DNA）和细胞核（740.0±364.9 fmol/mg DNA）孕酮受体浓度也减少。显然，分泌颗粒的产生和孕酮受体浓度在时间上依赖于雌二醇。
