Geffard M, Buijs R M, Seguela P, Pool C W, Le Moal M
Brain Res. 1984 Feb 27;294(1):161-5. doi: 10.1016/0006-8993(84)91323-4.
Dopamine was coupled to bovine serum albumin (BSA) with glutaraldehyde, precautions were taken in order to preserve the catechol ring. After injection of this immunogen into rabbits, anti-dopamine antibodies were obtained and tested using radioimmunochemical binding studies and adsorption to catecholamine covered sepharose beads. A good correlation was found between the results of the different test systems, allowing us to visualize dopamine specifically in glutaraldehyde-fixed rat brains.
多巴胺通过戊二醛与牛血清白蛋白（BSA）偶联，采取了预防措施以保护儿茶酚环。将这种免疫原注射到兔子体内后，获得了抗多巴胺抗体，并使用放射免疫化学结合研究和对儿茶酚胺包被的琼脂糖珠的吸附进行了测试。在不同测试系统的结果之间发现了良好的相关性，这使我们能够在戊二醛固定的大鼠脑中特异性地可视化多巴胺。
