Zerwekh J E, Nicar M J
Clin Chem. 1984 Mar;30(3):452-3.
We describe the performance of a colorimetric reagent kit (Diagnostic Systems Laboratories, Inc., Webster, TX 77598) for measuring calcium directly in urine, serum, and ultrafiltered serum, and compare the results with those obtained with atomic absorption spectrophotometry. The CV for within-run precision was 2.8 and 2.1% for urine and whole serum, respectively (n = 10 each). Between-run precision for urine, whole serum, and ultrafiltered serum was 1.9, 1.6, and 2.2%, respectively (n = 8 to 10). Analytical recovery of added calcium from three different urine and serum specimens, to which three different concentrations of calcium had been added, was 101.9 (SD 0.3%) for urine and 100.9 (SD 0.2%) for serum. Assay of 30 urine specimens, 15 ultrafiltered serums, and 20 whole serums by both the kit and atomic absorption spectrophotometry demonstrated correlation coefficients of 0.993, 0.828, and 0.751, respectively. Mg2+, hemolysis, or lipemia does not interfere. Compared with atomic absorption spectrophotometry, the calcium kit procedure is rapid and simple.
我们描述了一种比色试剂试剂盒（诊断系统实验室公司，得克萨斯州韦伯斯特，邮编77598）用于直接测定尿液、血清和超滤血清中钙的性能，并将结果与原子吸收分光光度法所得结果进行比较。批内精密度的变异系数（CV），尿液和全血清分别为2.8%和2.1%（每组n = 10）。尿液、全血清和超滤血清的批间精密度分别为1.9%、1.6%和2.2%（n = 8至10）。对添加了三种不同浓度钙的三种不同尿液和血清标本进行添加钙的分析回收率，尿液为101.9（标准差0.3%），血清为100.9（标准差0.2%）。用该试剂盒和原子吸收分光光度法对30份尿液标本、15份超滤血清和20份全血清进行检测，相关系数分别为0.993、0.828和0.751。镁离子、溶血或脂血均无干扰。与原子吸收分光光度法相比，该钙试剂盒方法快速且简便。
