Kim M, Kim Y, Azar S H, Jeraj K, Michael A F
Clin Immunol Immunopathol. 1984 Mar;30(3):393-404. doi: 10.1016/0090-1229(84)90025-4.
The kinetics of radiolabeled heat-aggregated human IgG (AHIgG125I) were studied in rats with passive Heymann's nephritis (PHN) induced 72 hr previously with decomplemented rabbit antiserum to rat FX1A. Control rats were injected with decomplemented normal rabbit serum (NRS). Following administration of AHIgG125I (40 mg per 100 g of body wt) control and FX1A animals were sacrificed in groups of five each at 2, 4, 8, 16, and 24 hr and kidney, liver, spleen, lung, plasma, and blood cells obtained. 131I-Labeled human serum albumin (HSA131I) was administered prior to sacrifice as a plasma marker. In FX1A rats the following observations were made in comparison with control rats: (1) A decrease in the concentration of AHIgG125I in glomeruli was observed at 2, 4, and 8 hr after administration; (2) a significant increase in clearance reflected by a decrease in the concentration of plasma trichloroacetic acid (TCA)-precipitable radioactivity, and AHIgG125I (greater than 7 S) was present; (3) a significant increase in non-TCA-precipitable radioactivity in plasma and blood cells at most time periods; and (4) decreased concentrations of AHIgG125I in liver and spleen but not lung. The specificity of these observations was supported in separate experiments by the lack of any difference in the plasma levels of TCA-precipitable radioactivity after administration of radiolabeled albumin to FX1A and control rats. Studies in FX1A and control rats revealed no differences in body weight, kidney weight, hematocrit, blood volume, urine output, glomerular filtration rate, renal blood flow, or renal vascular resistance. A slight increase in urinary rat albumin excretion was observed in FX1A rats. The lower values of AHIgG125I observed in plasma, liver, and spleen associated with increased levels of non-TCA-precipitable radioactivity in plasma and blood cells suggest enhanced catabolism of AHIgG125I in FX1A rats, leading to decreased localization within the mesangium.
用已去除补体的兔抗大鼠FX1A抗血清于72小时前诱导产生被动型海曼肾炎（PHN）的大鼠，研究放射性标记的热聚集人IgG（AHIgG125I）的动力学。对照大鼠注射已去除补体的正常兔血清（NRS）。给予AHIgG125I（每100克体重40毫克）后，分别于2、4、8、16和24小时，将对照和FX1A组的动物每组5只处死，并获取肾脏、肝脏、脾脏、肺、血浆和血细胞。在处死前给予131I标记的人血清白蛋白（HSA131I）作为血浆标志物。与对照大鼠相比，在FX1A大鼠中观察到以下情况：（1）给药后2、4和8小时，肾小球中AHIgG125I的浓度降低；（2）清除率显著增加，表现为血浆三氯乙酸（TCA）可沉淀放射性以及AHIgG125I（大于7S）浓度降低；（3）在大多数时间段，血浆和血细胞中非TCA可沉淀放射性显著增加；（4）肝脏和脾脏中AHIgG125I浓度降低，但肺中未降低。在单独实验中，给FX1A大鼠和对照大鼠注射放射性标记白蛋白后，血浆中TCA可沉淀放射性水平没有差异，支持了这些观察结果的特异性。对FX1A大鼠和对照大鼠的研究表明，它们在体重、肾脏重量、血细胞比容、血容量、尿量、肾小球滤过率、肾血流量或肾血管阻力方面没有差异。在FX1A大鼠中观察到尿大鼠白蛋白排泄略有增加。血浆、肝脏和脾脏中AHIgG125I值较低，同时血浆和血细胞中非TCA可沉淀放射性水平升高，这表明FX1A大鼠中AHIgG125I的分解代谢增强，导致系膜内定位减少。
