Feld L G, Langford D J, Schwartz G J
Clin Pediatr (Phila). 1984 Mar;23(3):154-6. doi: 10.1177/000992288402300304.
Bilirubin interferes with the measurement of plasma creatinine by the kinetic colorimetric Jaffe reaction, the method currently used by many hospital laboratories. The purpose of the present study was to evaluate the influence of unconjugated bilirubin on the measurement of plasma creatinine by the AutoAnalyzer Jaffe end point method. This colorimetric method is slower but more precise and accurate in the range of 0.25 to 0.5 mg/dl. We found that bilirubin from jaundiced neonates and from stock solutions did not affect the determination of creatinine chromogen in plasma or in saline, even at concentrations as high as 25 mg/dl. We conclude that the use of the Jaffe end point AutoAnalyzer method with a blank run before each sample will provide an accurate measurement of creatinine in the plasma of the neonate with unconjugated hyperbilirubinemia.
胆红素会干扰通过动力学比色法Jaffe反应测定血浆肌酐，这是目前许多医院实验室使用的方法。本研究的目的是评估未结合胆红素对自动分析仪Jaffe终点法测定血浆肌酐的影响。这种比色法较慢，但在0.25至0.5mg/dl范围内更精确、准确。我们发现，来自黄疸新生儿和储备溶液的胆红素即使在浓度高达25mg/dl时，也不会影响血浆或盐水中肌酐色原的测定。我们得出结论，在每个样本之前进行空白运行的Jaffe终点自动分析仪方法将能够准确测量患有未结合型高胆红素血症的新生儿血浆中的肌酐。
