Knapp M L, Hadid O
Ann Clin Biochem. 1987 Jan;24 ( Pt 1):85-97. doi: 10.1177/000456328702400114.
Negative interference by jaundiced plasma in kinetic Jaffé methods for plasma creatinine determination is caused by both conjugated and unconjugated bilirubin. The amount of interference observed is method- and temperature-dependent and is directly related to bilirubin concentration but is independent of the creatinine concentration. Bilirubin also significantly reduces the precision of the creatinine estimation. A mechanism of bilirubin interference in kinetic Jaffé methods is proposed and the influence of picrate concentration, hydroxide concentration and temperature on the magnitude of bilirubin interference has been investigated.
黄疸血浆对动力学Jaffé法测定血浆肌酐产生的负干扰是由结合胆红素和非结合胆红素共同引起的。观察到的干扰量取决于方法和温度，与胆红素浓度直接相关，但与肌酐浓度无关。胆红素还会显著降低肌酐估计值的精密度。本文提出了胆红素在动力学Jaffé法中产生干扰的机制，并研究了苦味酸盐浓度、氢氧化物浓度和温度对胆红素干扰程度的影响。
