Mulholland M W, Quigley T, Bonsack M, Delaney J P
Endocrinology. 1984 Mar;114(3):840-4. doi: 10.1210/endo-114-3-840.
Using a quantitative immunocytochemical technique, antral gastrin cell populations in the rat were studied in various states of thyroid function. Simultaneous determinations of circulating serum gastrin were made by RIA. Rats made hypothyroid by ingestion of methimazole (0.01% solution in drinking water for 30 days) demonstrated a significant 32% decrease in gastrin cell density (306 +/- 9/cm vs. 207 +/- 11/cm for controls) associated with a significant 50% decrease in serum gastrin (143 +/- 12 vs. 307 +/- 20 pg/ml for controls). Induction of hypothyroidism and hypoparathyroidism by surgical thyroparathyroidectomy resulted in similarly significant decreases in gastrin cell numbers (229 +/- 12/cm) and serum gastrin (169 +/- 14 pg/ml). Animals that underwent thyroparathyroidectomy followed by T4 replacement (2.5 micrograms/100 g X day, ip) for 30 days had a mean gastrin cell density that was not significantly different from that of controls; serum gastrin was decreased to 207 +/- 11 pg/ml. The administration of excess T4 (200 micrograms/100 g X day, ip) for either 15 or 30 days was associated with a significant increase in gastrin cell numbers (413 +/- 23/cm at 15 days; 352 +/- 21/cm at 30 days). Mean serum gastrin was increased by 82% after 15 days of T4 administration (558 +/- 51 pg/ml) and by 65% at 30 days (506 +/- 36 pg/ml). We conclude that T4 is trophic for gastrin cells in the rat.
采用定量免疫细胞化学技术，研究了处于不同甲状腺功能状态下大鼠胃窦部胃泌素细胞群。通过放射免疫分析法同步测定循环血清胃泌素。通过摄入甲巯咪唑（饮用水中0.01%溶液，持续30天）造成甲状腺功能减退的大鼠，其胃泌素细胞密度显著降低32%（对照组为306±9个/厘米，实验组为207±11个/厘米），同时血清胃泌素显著降低50%（对照组为307±20皮克/毫升，实验组为143±12皮克/毫升）。通过手术切除甲状腺和甲状旁腺诱导甲状腺功能减退和甲状旁腺功能减退，导致胃泌素细胞数量（229±12个/厘米）和血清胃泌素（169±14皮克/毫升）同样显著降低。接受甲状腺甲状旁腺切除术后接受T4替代治疗（2.5微克/100克×天，腹腔注射）30天的动物，其平均胃泌素细胞密度与对照组无显著差异；血清胃泌素降至207±11皮克/毫升。连续15天或30天给予过量T4（200微克/100克×天，腹腔注射）与胃泌素细胞数量显著增加相关（15天时为413±23个/厘米；30天时为352±21个/厘米）。给予T4 15天后平均血清胃泌素增加82%（558±51皮克/毫升），30天时增加65%（506±36皮克/毫升）。我们得出结论，T4对大鼠胃泌素细胞具有营养作用。
