Haggerty R M, John D T
Infect Immun. 1978 Apr;20(1):73-7. doi: 10.1128/iai.20.1.73-77.1978.
The mouse system provides an excellent model for studying host resistance to Naegleria fowleri, the agent of primary amoebic meningoencephalitis. Innate resistance to infection with N. fowleri was examined with respect to infecting dose and the age, sex, and strain of mice. Intravenous inoculation with 10(7) amoebae per mouse produced 100% mortality in 9 days, whereas inoculation with fewer amoebae reduced the cumulative mortality. Male and female DUB/ICR mice of varying ages were inoculated intravenously with 2.5 X 10(5) N. fowleri per g of body weight. The youngest mice died first, with 100% mortality for both males and females, and mortality decreased with increasing age. Female mice were significantly more resistant to infection than males. Five strains of mice weighing approximately 20 g were inoculated intravenously with weight-adjusted doses; mortality ranged from 10% in C57BL/6 mice to 95% in A/HeCr mice.
小鼠系统为研究宿主对原发性阿米巴脑膜脑炎病原体福氏耐格里阿米巴的抵抗力提供了一个极佳的模型。针对感染剂量以及小鼠的年龄、性别和品系，研究了对福氏耐格里阿米巴感染的先天抵抗力。每只小鼠静脉接种10⁷个阿米巴原虫，9天内死亡率达100%，而接种较少的阿米巴原虫则降低了累积死亡率。不同年龄的雄性和雌性DUB/ICR小鼠按每克体重静脉接种2.5×10⁵个福氏耐格里阿米巴。最年幼的小鼠最先死亡，雄性和雌性的死亡率均为100%，且死亡率随年龄增长而降低。雌性小鼠对感染的抵抗力明显强于雄性。对5个品系体重约20克的小鼠按体重调整剂量进行静脉接种；死亡率从C57BL/6小鼠的10%到A/HeCr小鼠的95%不等。
