Zorzano A, Herrera E
Int J Biochem. 1984;16(3):263-7. doi: 10.1016/0020-711x(84)90098-3.
Circulating [14C]glucose 2, 5 and 10 min after intravenous injection of [U-14C]-L-alanine was greater in 24 hr starved than in fed rats. In vitro uptake of [14C]alanine by liver and kidney cortex slices from 24 hr starved and fed rats rose in parallel with increased medium substrate concentration. Formation of [14C]glucose from 1mM [14C]alanine was similar in liver and kidney cortex slices and increased in tissues from 24 hr starved compared with fed rats. With 5 mM [14C]alanine more [14C]glucose was produced by liver than by kidney cortex slices from 24 hr starved rats. Liver slices always produced more [14C]lactate and less [14C]-CO2 from [14C]alanine than kidney cortex slices. It is proposed that under physiological conditions, the kidneys cortex actively participates in glucose production from alanine.
静脉注射[U-¹⁴C]-L-丙氨酸后2、5和10分钟，24小时饥饿大鼠的循环[¹⁴C]葡萄糖水平高于喂食大鼠。来自24小时饥饿和喂食大鼠的肝和肾皮质切片对[¹⁴C]丙氨酸的体外摄取与培养基底物浓度的增加呈平行上升。1mM [¹⁴C]丙氨酸生成[¹⁴C]葡萄糖的情况在肝和肾皮质切片中相似，与喂食大鼠相比，24小时饥饿大鼠组织中的生成量增加。对于5mM [¹⁴C]丙氨酸，24小时饥饿大鼠的肝脏切片产生的[¹⁴C]葡萄糖比肾皮质切片多。肝切片从[¹⁴C]丙氨酸产生的[¹⁴C]乳酸总是比肾皮质切片多，而产生的[¹⁴C]-CO₂比肾皮质切片少。有人提出，在生理条件下，肾皮质积极参与丙氨酸生成葡萄糖的过程。
