Zorzano A, Herrera E
Int J Biochem. 1986;18(7):583-7. doi: 10.1016/0020-711x(86)90237-5.
The appearance of plasma [14C]glucose in the inferior cava vein after a pulse of 0.2 mmol of [U-14C]L-alanine or [U-14C]glycerol/200 g body wt given through the portal vein was studied in fed 21 day pregnant rats and virgin controls under pentobarbital anesthesia. In both groups values were much higher when [U-14C]glycerol was the administered tracer than when [U-14C]L-alanine, and they were augmented in pregnant versus virgin animals at 1 min when receiving [U-14C]glycerol and at 2 min when receiving [U-14C]L-alanine. 20 min after the tracers rats receiving [U-14C]glycerol showed much higher liver [14C]glycogen and [14C]glyceride glycerol than those receiving [U-14C]L-alanine. Radioactivity present in liver as [14C]glyceride glycerol was greater in pregnant than in virgin rats receiving [U-14C]glycerol whereas radioactivity corresponding to [14C]fatty acids was lower in the former group receiving either tracer. At 20 min after maternal treatments fetuses showed lower plasma [14C]glycerol than [14C]alanine values but plasma [14C]glucose and liver [14C]glycogen values were much greater in fetuses from mothers receiving [U-14C]glycerol than [U-14C]L-amine. Besides showing the higher gluconeogenic efficiency in pregnant than in virgin rats, results indicate that at late gestation glycerol is used as a preferential substrate for both glucose and glyceride glycerol synthesis in liver.
在戊巴比妥麻醉下，对21日龄的妊娠大鼠和未孕对照大鼠进行研究，通过门静脉给予0.2 mmol/200 g体重的[U-14C]L-丙氨酸或[U-14C]甘油脉冲后，观察下腔静脉中血浆[14C]葡萄糖的出现情况。在两组中，当给予[U-14C]甘油作为示踪剂时的值远高于给予[U-14C]L-丙氨酸时，并且在接受[U-14C]甘油的1分钟时以及接受[U-14C]L-丙氨酸的2分钟时，妊娠动物的值相对于未孕动物有所增加。给予示踪剂20分钟后，接受[U-14C]甘油的大鼠肝脏中的[14C]糖原和[14C]甘油三酯甘油比接受[U-14C]L-丙氨酸的大鼠高得多。接受[U-14C]甘油的妊娠大鼠肝脏中以[14C]甘油三酯甘油形式存在的放射性比未孕大鼠高，而接受任何一种示踪剂的前一组中与[14C]脂肪酸相对应的放射性较低。母体处理后20分钟，胎儿血浆中的[14C]甘油值低于[14C]丙氨酸值，但接受[U-14C]甘油的母亲所生胎儿的血浆[14C]葡萄糖和肝脏[14C]糖原值比接受[U-14C]L-丙氨酸的母亲所生胎儿高得多。结果表明，妊娠大鼠的糖异生效率高于未孕大鼠，并且在妊娠后期，甘油被用作肝脏中葡萄糖和甘油三酯甘油合成的优先底物。
