在培养中培养小鼠乳腺上皮细胞。
To grow mouse mammary epithelial cells in culture.
作者信息
Ehmann U K, Peterson W D, Misfeldt D S
出版信息
J Cell Biol. 1984 Mar;98(3):1026-32. doi: 10.1083/jcb.98.3.1026.
Abstract
Normal mouse mammary epithelial cells from Balb/c mice were successfully cultivated on tissue culture plastic with lethally irradiated LA7 feeder cells. The feeder cells also promoted colony formation from single mouse mammary cells, and the fraction of cells that formed colonies was proportional to the density of feeder cells. The mouse mammary cells could be passaged at least 8-12 times as long as new feeder cells were added at each passage. The cells now in culture have doubled in number at least 30 times, but the in vitro lifespan is not yet known. The cultures of mouse cells maintained by this technique never became overgrown with fibroblasts and numerous domes formed in the cultures.
摘要
来自Balb/c小鼠的正常乳腺上皮细胞在经致死剂量照射的LA7饲养细胞存在的情况下，成功地在组织培养塑料板上培养出来。饲养细胞还促进了单个小鼠乳腺细胞的集落形成，并且形成集落的细胞比例与饲养细胞的密度成正比。只要在每次传代时添加新的饲养细胞，小鼠乳腺细胞就可以传代至少8 - 12次。目前培养的细胞数量至少已经翻倍30次，但体外寿命尚不清楚。用这种技术维持的小鼠细胞培养物从未被成纤维细胞过度生长，并且在培养物中形成了许多圆顶状结构。
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本文引用的文献
1
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J Cell Biol. 1980 Oct;87(1):255-63. doi: 10.1083/jcb.87.1.255.
2
Clonal growth of normal and malignant human breast epithelia.
J Surg Oncol. 1981;18(3):317-22. doi: 10.1002/jso.2930180313.
3
Clonal growth of normal adult human bronchial epithelial cells in a serum-free medium.正常成人支气管上皮细胞在无血清培养基中的克隆生长。
In Vitro. 1982 Jul;18(7):633-42. doi: 10.1007/BF02796396.
4
Clonal proliferation of cultured nonmalignant and malignant human breast epithelia.培养的非恶性和恶性人乳腺上皮细胞的克隆增殖。
Cancer Res. 1981 Nov;41(11 Pt 1):4637-43.
5
Growth of human mammary epithelial cells on collagen gel surfaces.人乳腺上皮细胞在胶原凝胶表面的生长。
Cancer Res. 1981 Oct;41(10):4093-100.
6
Methods for growth of cultured cells in serum-free medium.在无血清培养基中培养细胞的方法。
Anal Biochem. 1980 Mar 1;102(2):255-70. doi: 10.1016/0003-2697(80)90151-7.
7
Nil-8 conditioned medium stimulates growth of human mammary epithelial cells.
Breast Cancer Res Treat. 1981;1(4):375-9. doi: 10.1007/BF01806754.
8
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Methods Cell Biol. 1980;21B:195-225. doi: 10.1016/s0091-679x(08)60684-6.
9
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