Snodgrass D R, Angus K W, Gray E W
J Comp Pathol. 1984 Jan;94(1):141-52. doi: 10.1016/0021-9975(84)90016-1.
Contaminating bacteria were removed from an isolate of calf Cryptosporidium by 3 sequential passages of the parasite in gnotobiotic lambs, together with antibiotic treatment of the lambs. This preparation, which contained no detectable bacteria or viruses, was given by mouth to 8 2-day-old gnotobiotic lambs, 3 of which were dosed at the same time with bacterial flora from a healthy calf. Lambs were killed at intervals from 12 to 288 h post-inoculation and the sequential development of the parasite, of enteric lesions, and of clinical illness was observed. Lesions were characterized by severe villus stunting and fusion. Clinically the most consistent sign was anorexia, with some lambs developing also a severe watery diarrhoea. Lesions and clinical signs were similar in lambs with and without intestinal bacteria. This demonstration of the enteropathogenicity of Cryptosporidium in germfree lambs suggests that it is a pathogen of significance.
通过将寄生虫在无菌羔羊中连续传代3次，并对羔羊进行抗生素治疗，从小牛隐孢子虫分离株中去除了污染细菌。这种不含可检测到的细菌或病毒的制剂经口给予8只2日龄的无菌羔羊，其中3只同时接种了健康小牛的菌群。在接种后12至288小时的不同时间点宰杀羔羊，观察寄生虫、肠道病变和临床疾病的连续发展情况。病变的特征是严重的绒毛发育不良和融合。临床上最一致的症状是厌食，一些羔羊还出现严重的水样腹泻。有无肠道细菌的羔羊的病变和临床症状相似。这种在无菌羔羊中隐孢子虫肠道致病性的证明表明它是一种重要的病原体。
