Danford N
Mutat Res. 1984 Mar;139(3):127-32. doi: 10.1016/0165-7992(84)90007-1.
The use of a short hypotonic treatment can prevent scattering of chromosomes by retaining them within the cell membrane. This technique allows chromosomes to be counted accurately so that hypodiploidy can be assessed as well as hyperdiploidy. Levels of aneuploidy were measured following treatment of a low passage number Chinese hamster fibroblast cell line derived from liver (CH1-L) with colcemid, resulting in a dose-related increase in both aneuploidy and polyploidy. The system may be a valuable assay to extend the biological profile of environmental agents, particularly those that are carcinogenic but do not induce chromosome breakage.
使用短时间的低渗处理可以通过将染色体保留在细胞膜内来防止其分散。该技术能够准确地对染色体进行计数，从而可以评估亚二倍体以及超二倍体。在用秋水仙酰胺处理源自肝脏的低传代中国仓鼠成纤维细胞系（CH1-L）后，测量了非整倍体水平，结果显示非整倍体和多倍体均呈剂量相关增加。该系统可能是一种有价值的检测方法，可用于扩展环境因子的生物学特征，特别是那些具有致癌性但不诱导染色体断裂的因子。
