Scheres J M
Cytogenet Cell Genet. 1977;18(1):2-12. doi: 10.1159/000130743.
A number of salt solutions were tested for their ability to replace 2 X SSC in the production of C-bands with the so-called CT technique. Those containing monovalent cations, except for the ones with Ag+ ions, were capable of inducing C-bands in Ba(OH)2-pretreated human chromosomes. Cesium cations were the most effective in this respect. In contrast to the effect of the monovalent cations studied, di- or polyvalent cations (and Ag+ cations) were incapable of producing C-bands, but appeared to stabilize the Ba(OH)2-induced reverse-banding pattern, This stabilizing effect was obtainable with relatively low cation concentrations and persisted even when C-band-producing cations were present at the same time. The experiments clearly show that the cations present during salt incubations, generally used in the CT- and several other banding methods, can exert a pronounced influence on chromosome behavior.
使用所谓的CT技术，测试了多种盐溶液在产生C带时替代2X SSC的能力。除含Ag +离子的溶液外，那些含有单价阳离子的溶液能够在经Ba(OH)2预处理的人类染色体中诱导产生C带。在这方面，铯阳离子最为有效。与所研究的单价阳离子的作用相反，二价或多价阳离子（以及Ag +阳离子）不能产生C带，但似乎能稳定Ba(OH)2诱导的反带模式。这种稳定作用在相对较低的阳离子浓度下即可获得，即使同时存在产生C带的阳离子时也依然存在。实验清楚地表明，在CT及其他几种显带方法中通常使用的盐温育过程中存在的阳离子，可对染色体行为产生显著影响。
