Scheres J M
Hum Genet. 1976 Jul 27;33(2):167-74. doi: 10.1007/BF00281891.
This paper describes a study of the role of certain cations in the alkaline pretreatment step used in the CT technique for chromosome band formation. Treatment of human chromosomes with ammonium bases or with the hydroxides of the monovalent alkali metals Na, K, or Li resulted in their rapid disintegration, unless very short treatment periods or diluted solutions were used. In the latter cases a subsequent staining produced a weak G-banding pattern. The chromosomes appeared to be much less sensitive to treatment with the hydroxides of the divalent alkaline earth metals Ba, Sr, Ca, and Mg. Staining after exposure to these hydroxides yielded R-banding patterns. The reduced alkali sensitivity of the chromosomes and the reverse banding pattern formation observed are probably the result of a chromatin stabilization by the divalent cations of the alkaline earth metals. It is proposed that not only in the R-band formation with the hydroxides of the alkaline earth metals but also in that obtained by other techniques, chromosome stabilization plays an important role.
本文描述了一项关于某些阳离子在CT技术用于染色体带形成的碱性预处理步骤中所起作用的研究。用铵碱或单价碱金属Na、K或Li的氢氧化物处理人类染色体，会导致它们迅速解体，除非使用非常短的处理时间或稀释溶液。在后一种情况下，随后的染色会产生较弱的G带模式。染色体似乎对二价碱土金属Ba、Sr、Ca和Mg的氢氧化物处理不太敏感。暴露于这些氢氧化物后进行染色产生R带模式。观察到的染色体碱敏感性降低和反向带模式形成可能是碱土金属二价阳离子使染色质稳定的结果。有人提出，不仅在使用碱土金属氢氧化物形成R带的过程中，而且在通过其他技术获得R带的过程中，染色体稳定都起着重要作用。
