[研究混合物中天然钴胺素的极谱法]
[Polarographic method of studying natural cobalamins in mixtures].
作者信息
Kuznetsov B A, Eliseev S A
出版信息
Prikl Biokhim Mikrobiol. 1984 Jan-Feb;20(1):139-42.
PMID:6701161
Abstract
By means of polarography and tree-angle scan voltammetry, AdoCbl, MeCbl and OH-Cbl were studied in mixtures and separately. The possibility to obtain a polarographic scope of quality for each of the natural cobalamins studied was shown. The ability of cobalamins to be adsorbed on a mercury electrode enables to increase the sensitivity of the method by conducting the accumulative adsorption from solutions with the concentration up to 1 microgram/ml.
摘要
通过极谱法和三角扫描伏安法，对腺苷钴胺、甲基钴胺和羟基钴胺进行了混合研究和单独研究。结果表明，对于所研究的每一种天然钴胺素，都有可能获得质量极谱范围。钴胺素在汞电极上的吸附能力使得通过从浓度高达1微克/毫升的溶液中进行累积吸附来提高该方法的灵敏度成为可能。
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