核糖体糖蛋白的存在。伴刀豆球蛋白A1对来自小麦胚芽的游离核糖体和膜结合核糖体的凝集作用。

The presence of ribosomal glycoproteins. Agglutination of free and membrane-bound ribosomes from wheat germ by concanavalin A1.

作者信息

Yoshida K

出版信息

J Biochem. 1978 Jun;83(6):1609-14. doi: 10.1093/oxfordjournals.jbchem.a132072.

DOI:10.1093/oxfordjournals.jbchem.a132072

Abstract

Free and membrane-bound ribosomes prepared from wheat germ and extensively washed with 1% Triton X100-sodium deoxycholate, formed large aggregates in the presence of concanavalin A. The ribosomal aggregates were detected by sucrose density gradient centrifugation and immunoprecipitation. SDS-polyacrylamide gel electrophoresis of ribosomal proteins indicated that four ribosomal glycoproteins (MW about 14,000 to 24,000) were present in both free and membrane-bound ribosomes. However, three glycoproteins (MW about 25,000 to 15,000) appear to be underglycosylated or lacking in free ribosomes.

摘要

从小麦胚芽中制备的游离核糖体和膜结合核糖体，用1% Triton X100 - 脱氧胆酸钠进行充分洗涤后，在伴刀豆球蛋白A存在的情况下形成大的聚集体。通过蔗糖密度梯度离心和免疫沉淀检测核糖体聚集体。核糖体蛋白的SDS - 聚丙烯酰胺凝胶电泳表明，游离核糖体和膜结合核糖体中均存在四种核糖体糖蛋白（分子量约为14,000至24,000）。然而，三种糖蛋白（分子量约为25,000至15,000）在游离核糖体中似乎糖基化不足或缺乏。