Cardenas J M, Richards T C, Gabourel L
J Cell Physiol. 1978 Aug;96(2):189-97. doi: 10.1002/jcp.1040960207.
Electrophoretic and immunofluorescence analysis were used to study the distribution of pyruvate kinase isozymes in the bovine kidney. Electrophoretic analysis demonstrated the presence of large amounts of K4 plus small amounts of K-M hybrids in cortical, medullary, and papillary sections cut from the kidney. Nearly all of the K-L hybrids seen in whole kidney extracts were found in cortical sections. Immunofluorescence of frozen sections revealed the presence of type L subunits in the tubules but the complete absence of this subunit type in flomeruli. Glomeruli do contain large quantities of pyruvate kinase isozymes, probably K4 and K-M hybrids, that cross-react with antibodies produced against type M pyruvate kinase. Type L-containing forms of pyruvate kinase and aldolase type B both appear to be found in cell types thought to be capable of catalyzing of gluconeogenesis, while type K pyruvate kinase and type A aldolase are found in predominantly glycolytic cell types of the kidney. Lactate dehydrogenase isozymic patterns appear to be less closely correlated with glycolytic versus gluconeogenic functions of the kidney but may be determined more directly by other metabolic functions.
采用电泳和免疫荧光分析方法研究丙酮酸激酶同工酶在牛肾中的分布。电泳分析表明，从肾脏切取的皮质、髓质和乳头部分存在大量的K4以及少量的K-M杂种。在全肾提取物中观察到的几乎所有K-L杂种都存在于皮质部分。冷冻切片的免疫荧光显示，肾小管中存在L型亚基，但肾小球中完全没有这种亚基类型。肾小球确实含有大量的丙酮酸激酶同工酶，可能是K4和K-M杂种，它们与针对M型丙酮酸激酶产生的抗体发生交叉反应。含L型的丙酮酸激酶和B型醛缩酶似乎都存在于被认为能够催化糖异生的细胞类型中，而K型丙酮酸激酶和A型醛缩酶则主要存在于肾脏的糖酵解细胞类型中。乳酸脱氢酶同工酶模式似乎与肾脏的糖酵解和糖异生功能的相关性较小，但可能更直接地由其他代谢功能决定。
