Wachsmuth E D
Curr Probl Clin Biochem. 1976;6:381-91.
Histochemical and immuno-histochemical studies on kidney sections of adult rats and rats at different stages of development were carried out to estimate enzyme concentrations in nephron segments. Aminopeptidase and alkaline phosphatase were found from 3 days before birth in proximal tubule cells, aldolase-B and aldolase-A in all the nephron and collecting duct. The concentration of the enzymes remained remarkably constant from the 3rd day after birth onwards, except for aldolase-A in the distal tubule cells. The concentration of aldolase-B was higher than of aldolase-A in the distal tubule cells. The concentration of aldolase-B was higher than of aldolase-A, in glomerula and proximal tubules, that of aldolase-A was higher than that of aldolase-B in the ascending thick part of the Henle loop and in the following parts of the nephron. This implies that the concentration of the enzymes is the limiting factor for the regulation of substrate hydrolysis and that the nephron when formed can function as efficiently as the nephron of the adult rat with respect to these enzymes.
对成年大鼠以及处于不同发育阶段的大鼠的肾脏切片进行了组织化学和免疫组织化学研究，以评估肾单位各节段中的酶浓度。在出生前3天，近端小管细胞中就发现了氨肽酶和碱性磷酸酶，在所有肾单位和集合管中发现了醛缩酶B和醛缩酶A。除了远端小管细胞中的醛缩酶A外，出生后第3天起酶的浓度保持显著恒定。在远端小管细胞中，醛缩酶B的浓度高于醛缩酶A。在肾小球和近端小管中，醛缩酶B的浓度高于醛缩酶A，在亨氏袢升支粗段及肾单位的后续部分，醛缩酶A的浓度高于醛缩酶B。这意味着酶的浓度是调节底物水解的限制因素，并且形成后的肾单位在这些酶方面的功能效率与成年大鼠的肾单位一样高。
