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采用一种适用于尿液、血浆、脑和脑脊液的简单气液色谱法测定犬尿氨酸。

Determination of kynurenine by a simple gas-liquid chromatographic method applicable to urine, plasma, brain and cerebrospinal fluid.

作者信息

Joseph M H

出版信息

J Chromatogr. 1978 Jul 1;146(1):33-41. doi: 10.1016/s0378-4347(00)81287-6.

DOI:10.1016/s0378-4347(00)81287-6
PMID:670356
Abstract

A simple, sensitive and specific method for the determination of kynurenine is described. This is based on alkaline cleavage of kynurenine, followed by solvent extraction, trifluoroacetylation and gas--liquid chromatography with electron capture detection. Using this method kynurenine has been determined in urine and plasma, and for the first time in brain and cerebrospinal fluid. Increases in kynurenine in brain, plasma and urine are demonstrated following tryptophan administration to man and rat.

摘要

本文描述了一种测定犬尿氨酸的简单、灵敏且特异的方法。该方法基于犬尿氨酸的碱性裂解，随后进行溶剂萃取、三氟乙酰化以及带电子捕获检测的气液色谱分析。使用此方法已测定了尿液和血浆中的犬尿氨酸，并且首次测定了脑和脑脊液中的犬尿氨酸。对人和大鼠给予色氨酸后，脑、血浆和尿液中的犬尿氨酸均有所增加。

相似文献

1
Determination of kynurenine by a simple gas-liquid chromatographic method applicable to urine, plasma, brain and cerebrospinal fluid.采用一种适用于尿液、血浆、脑和脑脊液的简单气液色谱法测定犬尿氨酸。
J Chromatogr. 1978 Jul 1;146(1):33-41. doi: 10.1016/s0378-4347(00)81287-6.
2
The determination of kynurenine by gas-liquid chromatography; evidence for its presence in rat brain [proceedings].用气液色谱法测定犬尿氨酸；其在大鼠脑中存在的证据[会议论文集]
Br J Pharmacol. 1977 Mar;59(3):525P.
3
Quantification of L-tryptophan and L-kynurenine by liquid chromatography/electron capture negative ion chemical ionization mass spectrometry.采用液相色谱/电子捕获负离子化学电离质谱法对L-色氨酸和L-犬尿氨酸进行定量分析。
Biol Mass Spectrom. 1994 Jan;23(1):27-32. doi: 10.1002/bms.1200230106.
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Determination of 3-hydroxykynurenine in human brain and plasma by high-performance liquid chromatography with electrochemical detection. Increased concentrations in hepatic encephalopathy.高效液相色谱-电化学检测法测定人脑中及血浆中的3-羟基犬尿氨酸。肝性脑病中浓度升高。
J Chromatogr. 1991 Apr 19;565(1-2):436-40. doi: 10.1016/0378-4347(91)80406-3.
5
Development and validation of a single analytical method for the determination of tryptophan, and its kynurenine metabolites in rat plasma.建立并验证一种用于测定大鼠血浆色氨酸及其犬尿氨酸代谢产物的单一分析方法。
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Jun 1;898:121-9. doi: 10.1016/j.jchromb.2012.04.030. Epub 2012 May 4.
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LC-MS/MS-based quantification of kynurenine metabolites, tryptophan, monoamines and neopterin in plasma, cerebrospinal fluid and brain.基于液相色谱-串联质谱法对血浆、脑脊液和脑组织中犬尿氨酸代谢物、色氨酸、单胺和新蝶呤的定量分析。
Bioanalysis. 2016 Sep;8(18):1903-17. doi: 10.4155/bio-2016-0111. Epub 2016 Aug 15.
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A single-run liquid chromatography mass spectrometry method to quantify neuroactive kynurenine pathway metabolites in rat plasma.一种用于定量大鼠血浆中神经活性犬尿氨酸途径代谢物的单次运行液相色谱-质谱法。
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Positive identification of kynurenine in rat and human brain [proceedings].大鼠和人类大脑中犬尿氨酸的阳性鉴定[会议论文集]
Biochem Soc Trans. 1978;6(1):123-6. doi: 10.1042/bst0060123.
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The determination of kynurenine in plasma.血浆中犬尿氨酸的测定。
Clin Chim Acta. 1975 Sep 1;63(2):197-204. doi: 10.1016/0009-8981(75)90163-1.
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Procedural difficulties in the gas-liquid chromatographic assay of the arylalkylamines.芳基烷基胺的气液色谱分析中的操作困难。
J Chromatogr. 1976 Jul 21;123(1):45-50. doi: 10.1016/s0021-9673(00)81100-0.

引用本文的文献

1
The biological interactions between kynurenine and AhR in melanocytes: in vitro studies.色氨酸与 AhR 在黑素细胞中的生物学相互作用：体外研究。
Amino Acids. 2023 Jul;55(7):947-954. doi: 10.1007/s00726-023-03279-0. Epub 2023 May 28.
2
Kynurenic acid as an antagonist of α7 nicotinic acetylcholine receptors in the brain: facts and challenges.犬尿酸作为大脑中α7 烟碱型乙酰胆碱受体的拮抗剂：事实与挑战。
Biochem Pharmacol. 2013 Apr 15;85(8):1027-32. doi: 10.1016/j.bcp.2012.12.014. Epub 2012 Dec 25.
3
L-alpha-aminoadipate inhibits kynurenate synthesis in rat brain hippocampus and tissue culture.
Neurochem Res. 1997 Jul;22(7):825-9. doi: 10.1023/a:1022035926832.
4
Proceeding of the British Pharmacological Society, Clinical Pharmacology Section, 16-18th December, 1980, University of London. Abstracts.英国药理学会临床药理学分会会议记录，1980年12月16日至18日，伦敦大学。摘要
Br J Clin Pharmacol. 1981 Apr;11(4):409P-439P. doi: 10.1111/j.1365-2125.1981.tb01148.x.
5
Studies on kynurenine in human cerebrospinal fluid: lowered levels in epilepsy.人体脑脊液中犬尿氨酸的研究：癫痫患者水平降低。
J Neural Transm. 1983;58(3-4):193-204. doi: 10.1007/BF01252805.
6
Kynurenine: penetration to the brain, effect on brain tryptophan and 5-hydroxytryptamine metabolism and binding to plasma albumin [proceedings].犬尿氨酸：对大脑的渗透、对脑内色氨酸和5-羟色胺代谢的影响以及与血浆白蛋白的结合[会议论文集]
Br J Pharmacol. 1979 Jul;66(3):483P-484P.
7
Brain tryptophan metabolism in schizophrenia: a post mortem study of metabolites of the serotonin and kynurenine pathways in schizophrenic and control subjects.精神分裂症患者的脑色氨酸代谢：对精神分裂症患者和对照受试者血清素和犬尿氨酸途径代谢物的尸检研究
Psychopharmacology (Berl). 1979 Apr 25;62(3):279-85. doi: 10.1007/BF00431959.