Pearson S J, Reynolds G P
Department of Biomedical Science, University of Sheffield, U.K.
J Chromatogr. 1991 Apr 19;565(1-2):436-40. doi: 10.1016/0378-4347(91)80406-3.
A simple and specific method was developed for the determination of 3-hydroxykynurenine in brain tissue and blood plasma using high-performance liquid chromatography with electrochemical detection. This involved an extraction procedure using strong cation-exchange columns and also permitted the determination of 3-hydroxyanthranilic acid in brain tissue. The method was applied to the investigation of post mortem brain tissue from patients with hepatic encephalopathy. Cortical 3-hydroxykynurenine concentrations were substantially increased in such patients above control values, providing evidence for a dysfunction of tryptophan metabolism in this disease.
开发了一种简单且特异的方法，用于使用高效液相色谱-电化学检测法测定脑组织和血浆中的3-羟基犬尿氨酸。这涉及使用强阳离子交换柱的提取程序，并且还能够测定脑组织中的3-羟基邻氨基苯甲酸。该方法应用于对肝性脑病患者死后脑组织的研究。此类患者皮质中的3-羟基犬尿氨酸浓度比对照值大幅升高，为该疾病中色氨酸代谢功能障碍提供了证据。
