通过电子显微镜观察组蛋白高度乙酰化对核小体颗粒的影响。

Influence of histone hyperacetylation on nucleosomal particles as visualized by electron microscopy.

作者信息

Bertrand E, Erard M, Gómez-Lira M, Bode J

出版信息

Arch Biochem Biophys. 1984 Feb 15;229(1):395-8. doi: 10.1016/0003-9861(84)90167-x.

Abstract

Recently, Bode et al. [J. Bode, M. Gómez-Lira, and H. Schröter (1983) Eur. J. Biochem. 130, 437-445] have observed that monomeric nucleosomal particles from butyrate-treated Namalva lymphoma cells display a distinct heterogeneity in their mobilities on a nondenaturing 4% polyacrylamide gel. They have proposed that histone hyperacetylation induces a conformational change in monomers that can be modulated by the presence of HMG 14/17. The electron microscopic analyses presented here support these proposals.

摘要

最近，博德等人[J. 博德、M. 戈麦斯 - 利拉和H. 施罗特（1983年）《欧洲生物化学杂志》130卷，437 - 445页]观察到，来自丁酸盐处理的纳马瓦淋巴瘤细胞的单体核小体颗粒在非变性4%聚丙烯酰胺凝胶上的迁移率表现出明显的异质性。他们提出，组蛋白高度乙酰化会诱导单体发生构象变化，这种变化可由HMG 14/17的存在进行调节。此处呈现的电子显微镜分析支持了这些观点。